动物性食品中4种β-兴奋剂的超高效液相色谱-串联质谱法测定  

Determination of 4 β-agonists in animal origin food by ultra performance liquid chromatography-tandem mass spectrometry

作　　者：王春民[1] 张秋萍[1] 吴春霞[1] 马晓艳[1] 李建[1] 

机构地区：[1]苏州市疾病预防控制中心,江苏苏州215004

出　　处：《中国卫生检验杂志》2016年第21期3064-3066,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的利用超高效液相色谱-串联质谱(UPLC-MS/MS)测定动物性食品中克伦特罗、莱克多巴胺、沙丁胺醇和特布他林。方法样品经葡萄糖醛甙酶/芳基硫酸酯酶酶解,乙酸乙酯提取,固相萃取小柱净化后UPLC-MS/MS测定。实验优化了液相条件、质谱参数和固相萃取前处理步骤。结果 4种瘦肉精在浓度为1.0μg/L^25.0μg/L时,线性关系较好,相关系数(r)为0.999。莱克多巴胺、克伦特罗、沙丁胺醇、特布他林的检出限均为0.5μg/kg。当加标水平为2.0μg/kg时,回收率为84%~105%。结论本法可用于实际样品的检测,样品中莱克多巴胺的检出率为5.0%。Objective To determine the residue of 4 β- agonists（ ractopamine,clenbuterol,salbutamol and terbutaline） in animal origin food by ultra high performance liquid chromatography- tandem mass spectrometry（ UPLC- MS / MS）. Methods The target analytes in samples were enzymatic hydrolyzed with gucuronidase / aryl sulfatase,and then extracted with ethyl acetate,and cleaned up with MCX SPE column,and finally separated and detected with UPLC- MS / MS. The liquid phase conditions,mass spectra parameters and solid phase extraction pretreatment procedure were optimized. Results Good linearity was obtained when the concentrations of the 4 β- agonists were within 1. 0 μg / L- 25. 0 μg / L,with r = 0. 999. The detection limits of ractopamine,clenbuterol,salbutamol,terbutaline quantification were 0. 5 μg / kg. The recoveries were within 84%- 105% at the concentration of 2. 0 μg / kg. Conclusion The method can be applied to the determination of 4 β- agonists in animal origin food and the detected rate of ractopamine in samples was 5. 0%.

关 键 词：超高效液相色谱-串联质谱法 动物性食品 瘦肉精 Β-兴奋剂 

分 类 号：O657.63[理学—分析化学]