布拉酵母菌与凝结芽孢杆菌混合固体发酵的培养基优化研究  

The optimization of culture medium for Saccharomyces boulardii and Bacillus coagulans in mixed solid fermentation

作　　者：张怡[1] 刘敬为 童应凯[1] 

机构地区：[1]天津农学院农学与资源环境学院生物技术实验室,天津300384

出　　处：《饲料工业》2016年第22期50-52,共3页Feed Industry

基　　金：天津农学院科技发展基金项目[2014N14]

摘　　要：文章对布拉酵母菌与凝结芽孢杆菌进行混合固体发酵,并通过正交试验研究,根据其表观现象观察和发酵产物的测定,确定了培养基的最佳配方和最适生长条件。通过正交试验得出,以固体培养基(包括麸皮、豆饼粉、玉米粉)为底料,加入4%葡萄糖、无蛋白胨、0.005%硫酸镁、0.1%磷酸二氢钾,5%接种量接入布拉酵母菌与凝结芽孢杆菌混合菌液,30℃培养48 h,布拉酵母菌与凝结芽孢杆菌混合固体发酵培养基能得到较高产量的布拉酵母菌和凝结芽孢杆菌。结果显示,在该条件下,布拉酵母菌与凝结芽孢杆菌能够以最佳的生长状态生长。In this paper, Saeeharomyees boulardii and Bacillus were mixed in solid fermentation, ac- cording to its apparent phenomenon and the determination of fermentation products by the orthogonal experiment. The optimal medium was determined and the optimum growth conditions were determined, which the solid medium （including wheat bran, soybean powder, corn powder） was as the base material, adding 4% glucose, without peptone, 0.005% MgSO4, 0.1% KH2PO4, 1% inoculation quantity of Sae- eharomyees boulardii and Bacillus cereus bacteria, 30℃ for 48 h training. Under the afore condition, Saeeharomyees boulardii and Bacillus eoagulans can grow to an optimal growth state.

关 键 词：布拉酵母菌 凝结芽孢杆菌 固体发酵 正交试验 

分 类 号：TQ92[轻工技术与工程—发酵工程]