蘘荷提取物抗氧化能力及抑菌作用  

Antioxidant and Antimicrobial Activities of Components from Zingiber mioga

作　　者：王玉荣[1] 邢韶芳 李钟洙 朴美子[3] 朴香兰[1] 

机构地区：[1]中央民族大学中国少数民族传统医学研究院,中国少数民族传统医学国家民委-教育部重点实验室,北京100081 [2]北京燕化医院,北京102500 [3]青岛农业大学食品科学与工程学院,山东青岛266109

出　　处：《中国实验方剂学杂志》2016年第24期59-63,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81274186);教育部“长江学者和创新团队发展”计划项目(IRT-13R63)

摘　　要：目的:以新鲜蘘荷Zingiber mioga花蕾为原料,探讨蘘荷花蕾不同提取部位的抗氧化作用,并筛选其有效抑菌部位。方法:蘘荷花蕾用甲醇提取浓缩,依次萃取得到石油醚、二氯甲烷、乙酸乙酯、正丁醇和水等5个萃取部位后,采用1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除模型评价不同萃取部位抗氧化能力,用打孔扩散法考察萃取部位对大肠埃希菌、沙门氏菌、金黄色葡萄球菌和铜绿假单胞菌的体外抑菌活性。结果:加热回流法提取蘘荷花蕾,提取率为3.1%,其中乙酸乙酯部位DPPH自由基清除能力最高,半数抑制率(IC50)达到(26.22±1.12)mg·L-1,对铜绿假单胞菌的抑菌圈达到(10±1)mm,石油醚部位抑菌活性最高,对金黄色葡萄球菌的最低抑菌浓度(MIC)最低,达到25.0 mg·L-1。结论:蘘荷花蕾各部位均有一定的抗氧化能力,综合抑菌效果比较石油醚部位>二氯甲烷部位>乙酸乙酯部位>甲醇提取物,为蘘荷花蕾抗氧化产品、抑菌剂的开发利用提供理论基础。Objective： To obtain the information about the antioxidant and antimicrobial activities of components from the fresh flower bud of Zingiber mioga. Method： The flower bud of Z. mioga was extracted and concentrated with methanol,and after extraction with petroleum ether,dichloromethane,ethyl acetate,n-butanol and water,the five fractions were evaluated for antioxidant activities by DPPH radical scavenging method and antibacterial activities on four strains（ Escherichia coli, Salmonella lignieres, Pseudomonas aeruginosa and Staphylococcus aureus） by agar disc diffusion method. Result： The extraction rate of Z. mioga flower bud was3. 1% in heating reflux method; the ethyl acetate extract showed the highest scavenging capacity on DPPH radical with IC50of（ 26. 22 ± 1. 12） mg·L- 1,and its inhibition zone on Pseudomonas aeruginosa was（ 10 ± 1） mm;petroleum ether fraction showed highest antibacterial activities,with minimal inhibitory concentration（ MIC） of25. 0 mg·L- 1on Staphylococcus aureus. Conclusion： All of fractions of the methanol extract from Z. mioga flower bud showed antioxidant abilities. Their potency of antibacterial activity was as follows： petroleum ether fraction dichloromethane fraction ethyl acetate fraction methanol extract,providing theoretical basis for the utilization and development of Z. mioga flower bud antioxidant and bacteriostatic products.

关 键 词：蘘荷 抗氧化活性 抑菌能力 

分 类 号：R282[医药卫生—中药学]