酿酒酵母合成异源单萜类化合物的研究进展  

Research advances of heterologous synthesis of monoterpenes in Saccharomyces cerevisiae

作　　者：孙明雪[1] 周景文[1] 

机构地区：[1]江南大学生物工程学院,江苏无锡214122

出　　处：《工业微生物》2016年第6期54-58,共5页Industrial Microbiology

基　　金：国家自然科学基金重点项目(31130043);国家自然科学基金(31000807);江苏省自然科学基金重点项目(BK2011004);江苏省自然科学基金(BK2010150)

摘　　要：单萜类化合物在食品、医药和工业等领域有重要的应用,具有可观的经济价值。随着合成生物学的日益发展,利用微生物作为细胞工厂合成单萜类化合物成为时下的研究热点。酿酒酵母是真核生物表达的模式菌株,其甲羟戊酸途径为单萜类化合物的合成提供直接前体,因此在酿酒酵母中构建异源单萜类化合物合成途径有较大优势。本文介绍了酿酒酵母细胞中异源单萜类化合物合成途径的构建。从甲羟戊酸途径代谢通量调控机制和融合酶调控酶催化反应效率两方面概述了酿酒酵母异源合成单萜类化合物的研究进展。Monoterpenes are widely used in food, medicine and industries, which have great economical values. With the development of synthetic biology, monoterpenes synthesis using microbial cell as factory have become a hot research topic. Saccharomyces cerevisiae is a model eukaryotic expression strain, mevalonate pathway provides precursors for the synthesis of monoterpenes, so it has great advantages to construct heterologous monoterpenes synthesis pathway. The construction of heterologous monoterpenes synthesis pathway in Saccharomyces cerevisiae was introduced. The research advances in heterologous expression of monoterpenes in Saccharomyces cerevisiae were reviewed from regulatory mechanisms of metabolic flux of mevalonate pathway and regulation of enzyme catalytic efficiency using fusion enzymes.

关 键 词：酿酒酵母 甲羟戊酸途径 单萜类化合物 代谢 融合酶 

分 类 号：TS261.11[轻工技术与工程—发酵工程]