三元超分子荧光增敏快速检测黄曲霉毒素M_1  

Rapid Detection of Aflatoxin M_1 by Ternary Supramolecular System-Based Fluorescence Enhancement

作　　者：马良[1] 张宇昊[1] 江涛[1] 苏敏[1] 涂春蓉 

机构地区：[1]西南大学食品科学学院

出　　处：《食品科学》2016年第24期197-202,共6页Food Science

基　　金：国家重点基础研究发展计划(973计划)项目(2013CB127803);国家自然科学基金青年科学基金项目(31301476)

摘　　要：根据黄曲霉毒素M_1(aflatoxin M_1,AFM_1)的荧光特性,研究β-环糊精(β-cyclodextrin,β-CD)及其衍生物(β-CDs)、金属盐(Hg^(2+))与AFM1形成的三元超分子体系及对AFM1荧光增强作用,并探索超分子包合物形成的机理。甲基-β-CD-Hg^(2+)-AFM_1三元体系中,当溶剂组成为体积分数20%甲醇溶液,Hg^(2+)、M-β-CD与AFM_1物质的量比分别为6.6×10~4∶1、5.80×10~5∶1时,荧光增强倍数可达到4.5倍。光谱法、热力学方法表明AFM1能进入M-β-CD空腔,从而减少荧光淬灭,增强荧光检测的灵敏度。利用Benesi-Hildebrand双倒数法和Van’t Hoff热力学方程研究超分子体系的包合常数与热力学常数,初步探讨超分子反应机理。该荧光增敏技术检测AFM_1,在0.01~2.00μg/L范围内分析线性良好,相关系数R^2为0.999 2,检出限为0.0026μg/L。这种衍生方法具有灵敏、简单、高效、经济等优点,乳品中除铁元素以外的大多数强化微量元素对测定无干扰作用,可应用于奶制品中AFM1的快速检测。The formation mechanism of ternary supra-molecular systems composed of aflatoxin M1（AFM1）,β-cyclodextrin or its derivatives（β-CDs） and Hg^2＋ was studied by measuring the native fluorescence of AFM1 and fluorescence changes of the β-CD-Hg^2＋-AFM1 system.The fluorescence intensity of the methyl-β-CD-Hg^2＋-AFM1 ternary supramolecular system in 20%（V/V） aqueous methanol with a molar ratio of Hg^2＋ to M-β-CD to AFM1 of 6.6 × 10^4：5.80 × 10^5：1 could be 4.5 times higher than that of the native fluorescence intensity of AFM1.It was proved that AFM1 entered the cavity of M-β-CD,leading to reduced fluorescence quenching and increased sensitivity of detection by spectroscopic and thermodynamic analyses.Supermolecular inclusion constants and thermodynamic constant were calculated and the superamolecular reaction mechanism was studied preliminarily by the Benesi-Hidebrand equation and Van＇t Hoff equation.Good linearity was observed in the AFM1 concentration range of 0.01–2.00 μg/L with a correlation coefficient of 0.999 2 and the detection limit of this method was 0.002 6 μg/L.The method could be used in the rapid detection of AFM1 in dairy products with high sensitivity.It was simple,efficient and economical without interference from most fortified trace minerals except Fe～（3＋） in dairy products.

关 键 词：黄曲霉毒素M1 超分子体系 荧光增强 快速检测 包合 

分 类 号：TS207.3[轻工技术与工程—食品科学]