金黄色葡萄球菌对超高压抗性的研究  

The Resistance of Staphylococcus aureus to High Hydrostatic Pressure

作　　者：韩洪玲[1] 董月[1] 高瑀珑[1] 袁先雯 冯燕玲[1] 朱蕾[1] 

机构地区：[1]南京财经大学食品科学与工程学院,江苏省现代粮食流通与安全协同创新中心,江苏高校粮油质量安全控制及深加工重点实验室,南京210023

出　　处：《食品工业》2016年第12期146-151,共6页The Food Industry

基　　金：江苏省自然科学基金(BK20131435);国家自然科学基金(31371864);高校自然科学研究项目(12KJB550003);粮食公益性行业科研专项(201413007-05);江苏高校优势学科建设工程资助项目(PADA)

摘　　要：对金黄色葡萄球菌（ATCC6538）经不同胁迫条件处理后的超高压抗性进行了研究。将培养至平衡期的ATCC6538进行胁迫处理（培养温度、酸碱度和渗透压）,利用Weibull模型拟合ATCC6538的超高压抗性,测定在400MPa作用下的抗性,计算该菌数量首次减少一个数量级所需的时间δ。结果表明,在15℃~45℃的培养温度胁迫下,随着温度的升高,ATCC6538的压力抗性显著增加（p〈0.05）;pH 5.5的酸胁迫120 min与pH 9.5的碱胁迫120 min可诱导ATCC6538超高压抗性显著增加;0.5%~10.0%的氯化钠胁迫条件下,随着氯化钠浓度的提高,ATCC6538对超高压的抗性呈增加趋势。采用Weibull模型能较好地拟合ATCC6538以不同的培养温度、酸碱度和渗透压胁迫后在超高压作用下的抗性曲线,ATCC6538在不同酸碱、培养温度和渗透压等胁迫环境下可诱导其超高压抗性的增加。Study the development of resistance response in Staphylococcus aureus ATCC6538 to high hydrostatic pressure （HHP） treatment （400 MPa） after the exposure to different stressful conditions that may be encountered in food-processing environments. Stationary growth phase cells of ATCC6538 were exposed to stressful conditions （incubation temperature, acid and alkaline pH and osmotic stress） and then the acquisition of resistance to HHP （400 MPa） was determined. A model based on the Weibull distribution was used to describe the HHP-resistance curves, and time for the first decimal reduction was calculated （O values）. With increasing growth temperatures from 15 ℃ up to 45 ℃, the experimental results showed that the pressure resistance of ATCC6538 increased markedly （p〈0.05）. The conditions resulting in the maximum development of cross resistance （tolerance to HHP） were pH 5.5 （120 min）, pH 9.5 （120 min）. ATCC6538 cells were exposed to acid （pH 2.5） and alkali （pH 12.5） elevated acid or alkali stress failed to provide them with cross-protection against HHP. It was indicated that the development of cross resistance to HHP resulted in an increasing trend with the increase of NaC1 concentration from 0.5% to 10.0%. Subjecting to the different incubation temperature, acid, alkaline pH and osmotic stress, the curves of resistance to HHP （400 MPa） in ATCC6538 were fitted using WeibuU model. It also had been demonstrated that it could develop resistance response to HHP （400 MPa） under different stressing conditions, such as acid and alkaline pH, elevated temperatures and high osmotic stress.

关 键 词：超高压 金黄色葡萄球菌 超高压抗性 胁迫 交叉保护作用 

分 类 号：TS201.3[轻工技术与工程—食品科学]