龋齿及牙周病患者与健康人群口腔微生态的比较研究  

Comparison of Oral Microbial Diversity in Healthy People and Patients with Dental Caries and Periodontal Disease

作　　者：史彦[1] 李成杰[2] 张明正[2] 王鑫[3] 杨少国[3] 孟凡景[3] 陈廷涛[3] 杨健[1] 

机构地区：[1]南昌大学附属口腔医院牙体牙髓科,江西南昌330006 [2]南昌大学第二临床医学院,江西南昌330006 [3]南昌大学转化医学研究院,江西南昌330031

出　　处：《口腔医学研究》2016年第12期1265-1268,共4页Journal of Oral Science Research

基　　金：国家自然科学基金地区基金项目(编号:81503364/31560264);江西省科技厅项目(编号:20151BBG70239)

摘　　要：目的:利用高通量测序技术比较健康人和口腔患者口腔微生物的组成和变化。方法:选取典型的龋齿和牙周病患者,收集受刺激的唾液和口咽样本并进行高通量测序,利用生物信息学分析和比较各样品之间的物种组成差异。结果:各组的平均操作分类单元(OTU)为315个,其中健康人口腔内硬壁菌(Firmicutes)、拟杆菌(Bacteroidetes)、蛋白菌(Proteobacteria)、放线杆菌(Actinobacteria)和梭杆菌(Fusobacteria)对应的OTU数目分别73、64、53、19和18个;此外,相对丰富度结果表明硬壁菌(Firmicutes)在儿童组中减少而在青年组和成年组中增多,而蛋白菌(Proteobacteria)在儿童组中增多而在青年组和成年组中减少。结论:年龄段和口腔疾病对牙齿的发育和口腔微生物的组成影响巨大,传统意义上的致病菌可能在口腔健康中同样发挥着维护口腔稳态的作用。Objective：To compare the microbial diversity of healthy people and oral patients with high-throughput sequencing method and to provide basic data for curing dental caries and periodontal diseases.Methods：The patients with typical dental caries and periodontal diseases were selected.Their stimulated whole saliva and oropharyngeal samples were collected for high-throughput sequencing analysis and the microbial diversity among samples were analyzed using bioinformatics.Results：The Venn results indicated that a mean of 315 operational taxonomic units（OTUs）was obtained,and 73,64,53,19 and 18common OTUs belonging to Firmicutes,Bacteroidetes,Proteobacteria,Actinobacteria and Fusobacteria were identified in healthy people.In addition,the relative richness results indicated that the Firmicute was reduced in children group and increased in youth group and adult group,while the Proteobacteria was increased in children group but reduced in youth group and adult group.Conclusion：The age bracket and oral disease had largely influenced the tooth development and microbial development in oral cavity,and the traditional ＂pathogenic bacteria＂ of Firmicutes,Proteobacteriaand Bacteroidetes might exert beneficial effects on oral health.

关 键 词：高通量测序 龋齿 牙周炎 微生物多样性 

分 类 号：R781[医药卫生—口腔医学]