HPLC-MS^n对普洱茶各萃取组分与茶褐素中化学成分的分析  

Analysis of chemical compounds of theabrowine from pu-erh tea using HPLC-MS^n

作　　者：郭雪梅[1] 梁玉红[1] 陈欣[1] 张梁[1] 

机构地区：[1]安徽农业大学茶树生物学与资源利用国家重点实验室,合肥230036

出　　处：《安徽农业大学学报》2016年第6期853-857,共5页Journal of Anhui Agricultural University

基　　金：国家自然科学基金青年项目(31201335);安徽省自然科学基金青年项目(1308085QC51)共同资助

摘　　要：普洱茶是一类具有多种保健功效的后发酵茶,茶褐素是其重要的功能成分。茶褐素是普洱茶在渥堆过程中形成的一类结构非常复杂的混合物。茶褐素的主要成分是一类黄烷-3-醇的微生物代谢产物,其中以Puerins I-VIII为代表。利用系统的有机溶剂萃取,获得普洱茶不同萃取物和茶褐素组分,对获得的组分进行多酚类成分分析,发现在茶褐素部位未检出EC、GC、ECG,其中可可碱含量明显高于其他萃取部位。釆用高效液相色谱-质谱联用法(LC-MS^n)对普洱茶各萃取组分和茶褐素组分进行成分分析,比较普洱茶茶褐素与其他萃取物之间的化学成分差异,发现了茶褐素部位的主要标志性代谢产物普洱茶素。Pu-erh tea is a kind of post-fermented tea products, the bioactive polyphenols and theabrownine ofwhich possess varieties of healthcare functions. Theabrownine is a type of structurally complicated tea pigments,which are formed during the piling fermentation of ripened pu-erh tea.The different fractions and theabrownine ofripened pu-erh tea were separated using organic solvents＇ extraction, and subsequently were compared polyphe-nols chemically using high-performance liquid chromatography. EC, GC and ECG were not detected in the the-abrownine, However, Theabrownine oftheabrownine is higher than other extractions.The puerines were identifiedas the special component in theabrownine by using high-performance liquid chromatography coupled mass spec-trum （LC-MSn）.

关 键 词：普洱茶 茶褐素 LC-MSn 成分分析. 

分 类 号：TS272.54[农业科学—茶叶生产加工]