解淀粉芽孢杆菌产凝乳酶发酵优化  

Optimizing of medium and fermentation conditions for milk-clotting enzyme produced by Bacillus amyloliquefaciens

作　　者：王楠[1,2] 苑畅[1] 汪瑞[1] 淳于彦洁 吴雅雯[1] 李金宝[1] 丁重阳[1,2] 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,江苏无锡214122 [2]江南大学粮食发酵工艺与技术国家工程实验室,江苏无锡214122

出　　处：《生物加工过程》2016年第6期54-60,共7页Chinese Journal of Bioprocess Engineering

基　　金："十二五"国家科技支撑计划(2013BAD18B02);国家星火计划重点项目(2015GA690004);江苏省重点研发计划(现代农业)项目(BE2015306);江苏省产学研合作项目(BY2015019-36)

摘　　要：对实验室前期筛选到的一株解淀粉芽孢杆菌Bacillus amyloliquefaciens JNU002进行培养基和培养条件优化。单因素实验确定最佳碳源为乳糖,除麸皮外,其他氮源对产酶无明显促进作用,KH2PO4和Ca CO3等对产酶有促进作用。通过正交试验确定最佳培养基成分为麸皮16 g/L、乳糖10 g/L、KH2PO41 g/L、Ca CO31 g/L,其中麸皮对解淀粉芽孢杆菌产凝乳酶影响较大。在发酵温度37℃、种子液培养时间18 h、接种量0.5%、500 m L摇瓶装液量100m L时,较适宜解淀粉芽孢杆菌产凝乳酶。优化后解淀粉芽孢杆菌产凝乳酶活力达到12 766 SU/m L,比优化前提高了3.5倍。We optimized of culture medium and fermentation conditions to produce milk-clotting enzyme produced by Bacillus amyloliquefaciens JNU002.The results of single factor experiments showed that the best carbon source was lactose;nitrogen source had no obvious promoting effect;KH2PO4 and CaCO3 had promoting effect on enzyme production.Through the orthogonal experiment, the optimum culture medium composition was obtained： bran 16 g/L,lactose 10 g/L, KH2PO4 1 g/L, CaCO3 1 g/L. Bran had great influence on milk-clotting enzyme production.The culture temperature was 37℃, incubation time of liquid seed was 18 h, inoculum amount was 0. 5%, the liquid volume 100 mL/500 mL.In the optimized medium and under the optimized culture condition, milk-clotting enzyme activity of Bacillus amyloliquefaciens JNU002 reached 12 766 SU/mL an increase of 3.5 times.

关 键 词：解淀粉芽孢杆菌 凝乳酶 优化 

分 类 号：TQ925[轻工技术与工程—发酵工程]