紫苏HPPR基因启动子的克隆及植物表达载体构建  

Cloning and Construction of the Plant Expression Vectors of HPPR Gene Promoter from Perilla frutescens

作　　者：郭宇[1] 郝磊[1] 吕晓玲[1] 赵雪[1] 王超[1] 何东[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《分子植物育种》2016年第2期382-388,共7页Molecular Plant Breeding

基　　金：国家863高技术研究发展计划(2007AA100401)资助

摘　　要：以前期获得的紫苏HPPR基因的c DNA序列为模板,通过设计特异性引物和染色体步移的方法分离出HPPR基因启动子,全长2 216 bp,以此研究HPPR基因启动子的结构及功能特点。生物信息学分析表明,该序列中存在TATA-box和CAAT-box等典型的真核生物启动子基本元件,以及对茉莉酸甲酯、生长素、水杨酸和脱落酸等植物激素应答相关的顺式作用元件,另外也存在非生物胁迫顺式作用元件。将HPPR启动子部分缺失序列替代p BI121载体中的Ca MV35S启动子,构建了与GUS融合的启动子元件缺失表达载体。本试验的研究为该启动子在今后紫苏转基因定向改良提供了有用的候选资源。The c DNA sequence of HPPR gene of Perilla frutescens was used as template. The primers according to the c DNA sequence of HPPR gene were designed and HPPR gene promoter from genomic DNA was obtained by genomic walking to study the substructure and function of the HPPR. It showed that the HPPR promoter region was 2 216 bp. Software splicing and sequence analysis showed that the HPPR promoter region contained several kinds of elements including some typical elements TATA-box, CAAT-box. Some cis-acting elements involved in response to plant hormone, including Methyl Jasmonate, auxin, salicylic acid, abscisic acid, et al. Besides, it also included some elements responding to abiotic stresses. After that, the genomic DNA fragments with partial deletion in HPPR promoter and GUS fusion expression vector were constructed by replacing Ca MV35 S promoter of p BI121. These results would provide a useful candidate resource for the transgenic improvement in Perilla frutescens.

关 键 词：紫苏 迷迭香酸 HPPR 启动子 序列分析 缺失片段 

分 类 号：S567.219[农业科学—中草药栽培]