植物乳杆菌Lp-S2直投式发酵剂及其离心、冷冻干燥工艺研究  

Study on centrifugal and freeze drying of Lactobacillus plantarum Lp-S2 DVS

作　　者：隋春光[1] 梁金钟[1] 王风青[1] 

机构地区：[1]哈尔滨商业大学,黑龙江哈尔滨150076

出　　处：《食品与机械》2016年第11期174-177,共4页Food and Machinery

基　　金：黑龙江省高校科技创新团队建设计划项目(编号:2010td04)

摘　　要：以高产γ-氨基丁酸的植物乳杆菌Lp-S2为出发菌株,研制直投式发酵剂。并对发酵剂离心工艺及冷冻干燥工艺进行优化。通过正交试验确定植物乳杆菌Lp-S2的离心工艺为:离心温度4℃,离心时间30min,转速6 000r/min,该条件下植物乳杆菌Lp-S2离心收得率为83.8%。通过单因素和正交试验进行冷冻保护剂的优化。冷冻保护剂的最佳组合是15%海藻糖,15%葡萄糖和10%脱脂乳粉。植物乳杆菌Lp-S2直投式发酵剂,冻干存活率为39.2%,最佳预冷时间为6h。直投式发酵剂活菌数达到1.5×1012 CFU/mL。Lactobacillus plantarum Lp-S2 which has the ability to produce gamma-aminobutyric acid, was used as starting strain to develop Direct vat set （DVS）. Then the centrifugation process and freeze-drying process were optimized. By orthogonal experiment the optimal conditions of centrifugation were identified as： centrifugal temperature 4 ℃, Time-consuming 30 minutes, centrifugal force 6 000 r/min. In this condition, the centrifugal recovery rate of Lactobacillus Lp-S2 is 83.8%. The cryoprotectant was optimized by single factor and orthogonal test. The best combination of cryoprotectants was 15% trehalose, 15% glucose and 10% skim milk powder. The survival rate of L. plantarum Lp-S2 Direct Vat Set reached 39.20/00. The optimum pre-eooling time was 6 h. The number of viable cells of the direct-acting starter was 1.5 × 10^12 CFU/mL.

关 键 词：直投式菌剂 植物乳杆菌 冷冻干燥 Γ-氨基丁酸 

分 类 号：TS201.3[轻工技术与工程—食品科学]