具有α-葡萄糖苷酶抑制性益生乳酸菌的筛选  

Screening of probiotic lactic acid bacteria withα-glucosidase inhibition activity

作　　者：吕嘉枥[1] 张军蒙 余芳[1] 田延楚 LV Jia-li ZHANG Jun-meng YU Fang TIAN Yan-chu(School of Food and Biological Engineering, Shaanxi University of Science Technology, Xi＇an 710021, China)

机构地区：[1]陕西科技大学食品与生物工程学院,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2017年第1期129-133,共5页Journal of Shaanxi University of Science & Technology

基　　金：陕西省科技厅科技统筹创新工程计划项目(2011KTCQ03-08);咸阳市科技计划项目(2013K06-09)

摘　　要：通过对从发酵果蔬中初筛得到的18株乳酸菌益生性能中的产酸、耐受人工胃肠液能力的研究,得出18株乳酸菌所产总酸为0.8%到3.2%之间,其中有2株植物乳杆菌的总酸在3%以上,3株乳酸菌在1%以下.模拟人体内环境进行人工胃液、肠液耐受性实验中,存活率低于20%的有3株菌株,其余15株菌存活率都处于20%以上的水平.接下来进行α-葡萄糖苷酶抑制性研究,最终检测到这18种益生乳酸菌对α-葡萄糖苷酶都有不同的抑制效果,从5.71%到37.48%不等,其中抑制率最高的乳双歧杆菌1号菌株达到了37.48%,最低的为鼠李糖乳杆菌1号为5.71%.最终筛选出乳双歧杆菌1号菌株、干酪乳杆菌2号菌株、植物乳杆菌2号菌株、未培乳酸菌1号菌株为具有良好益生特性与α-葡萄糖苷酶抑制性的4株益生乳酸菌.The eighteen lactic acid bacteria strains which preliminary screening from the fer-mentation of fruits and vegetables, by research on their prebiotic properties of acid production ability and withstand artificial gastrointestinal fluid ability, we found that total acid amount produced by 18 lactic acid bacteria strains was between 0.8 % to 3. 2% , Which have two lactic acid bacteria strains total acid amount more than 3 % , three lactic acid bacteria strains were below 1%. In the research of tolerance with simulation environment of the human artificial gastric juice and intestinal juice test,we found that the survival rate of three strains was less than 20%,the remaining 15 strains survival rate are more than 20%. Then was a-glucosidase inhibition study,we finally detected that this 18 lactic acid bacteria strains have different a~ glucosidase inhibition activity, ranging from 5. 7 1 % to 37. 48% , the highest inhibition rate was Bifidobacterium animalis subsp. lactis No. 1 which reached 37. 48 % ？the lowest for Lac-tobacillus rhamnosus No. 1 was 5. 71%. Finally we selected four probiotic lactic acid bacteria strains have good probiotic properties and glucosidase inhibition activity which were Bifidobacterium animalis subsp. lactis No. 1 L a c to b a c i l lu s casei No. 2 L a c to b a c i l lu s p la n tarum No. 2 ^Uncul tured Lactobacillus No. 1.

关 键 词：乳酸菌 人工胃液 人工肠液 Α-葡萄糖苷酶 

分 类 号：O939.99[理学]