乳中阪崎克罗诺杆菌的快速捕获方法的建立  

Development on rapid capturing Cronobacter sakazakii in dairy products

作　　者：周文琦[1] 王蕊[1] 王喆[1] 丁木 赵玥明 满朝新 姜毓君[1,2] ZHOU Wenqi WANG Rui WANG Zhe DING Mu ZHAO Yueming MAN Chaoxin JIANG Yujun(Northeast Agricultural UniversiW Key Laboratory of Dairy Science, Ministry of Education, College of Food Science and Engineering Harbin 150030, China National Research Center for Dairy Engineering and Technology, Harbin 150028, China)

机构地区：[1]东北农业大学食品学院乳品科学教育部重点实验室,哈尔滨150030 [2]国家乳业工程技术研究中心,哈尔滨150028

出　　处：《中国乳品工业》2016年第12期19-21,共3页China Dairy Industry

基　　金：国家科技支撑计划课题(2013BAD18B11);黑龙江省杰出青年科学基金(JC201415);黑龙江省科研机构创新能力提升专项计划项目(YC13D005)

摘　　要：利用免疫磁捕获技术,建立了高效、快速的乳中阪崎克罗诺杆菌的捕获方法。将阪崎克罗诺杆菌抗体与Fe_3O_4磁珠偶联,制备特异性免疫磁珠并快速富集乳中的阪崎克罗诺杆菌。结果表明:此方法特异性强,且针对纯培养物中阪崎克罗诺杆菌的检测灵敏度达到104~10~5m L^(-1),乳中阪崎克罗诺杆菌的灵敏度达到10_5~10~6m L^(-1)。该方法能够大大缩短阪崎克罗诺杆菌的增菌时间,辅以适宜的检测方法即可实现对乳中阪崎克罗诺杆菌的快速富集,并且具有更高的特异性。Based on immune-magnetic capturing technology, a rapid and effective method to capture Cronobacter sakazakii in powdered in-fant formula was established in this paper. The antibody of Cronobacter sakazakii was coupling to the Fe304 magnetic particles to prepare forthe immuno-magnetic particles. The results showed this method was able to capture the Cronobacter sakazakii with a concentration of as lowas 10^5-10^6mL-1, and 10^5-10^6mL-1 in dairy product. Using this method, the enrichment period of detection can be shortened in a largescale. In assistance of suitable detection method, the rapid and accurate detection of Cronobacter sakazakii in dairy product can be accomplished.

关 键 词：免疫磁珠 阪崎克罗诺杆菌 快速富集 

分 类 号：Q93-335[生物学—微生物学]