模拟移动色谱连续分离大豆低聚糖技术研究  

Continuous Separation Technology of Oligosaccharide in Soybean by SMB

作　　者：陈洪生[1] 刁静静[2] 李洪飞[2] 李书海[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]黑龙江八一农垦大学国家杂粮工程技术研究中心,黑龙江大庆163319

出　　处：《中国食品学报》2016年第10期87-92,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：黑龙江省农垦总局科技攻关项目(HNK12A-04-10);黑龙江省大学生创新训练项目

摘　　要：以预处理后的大豆乳清废液为原料,采用模拟移动色谱分离系统对大豆低聚糖进行纯化。以大豆低聚糖纯度和回收率为指标,对进料量、进料流速、解吸流速、循环量流速等技术参数进行优化,结果表明,在进料量15%,进样流速5 m L/min,切换时间720 s,解吸流速10 m L/min,循环量流速13 m L/min条件下,获得的大豆低聚糖纯度为89%,收率在89%以上。This study investigated the optimal extractive condition of oligosaccharide from soybean processing liquid waste by self-made simulated moving bed（SMB） chromatography. Some factors were considered to evaluate purification effect including different feeding concentration, flow velocity, switching time, desorption velocity and circle velocity by using oligosaccharide purity and yield as purification indicators. The results indicated when feeding concentration was15%, flow velocity was 5 m L/min, switching time was 720 s, desorption velocity was 10 m L/min and circle velocity was13 m L/min, 89% soybean oligosaccharide was obtained and yield was over 89%.

关 键 词：模拟移动色谱 分离 大豆低聚糖 

分 类 号：TS214[轻工技术与工程—粮食、油脂及植物蛋白工程] TS201.2[轻工技术与工程—食品科学与工程]