谷氨酸棒杆菌代谢副产物对α-酮戊二酸积累的影响  

Effects of metabolic byproducts on accumulation of α-ketoglutarate in Corynebacterium glutamicum

作　　者：赵岩[1] 黄龙辉[1] 李娟[1] 孙兰超 李燕军[1,2,3] 谢希贤[1,2,3] 陈宁[1,2,3] ZHAO Yan HUANG Longhui LI Juan SUN Lanchao LI Yanjun XIE Xixian CHEN Ning(College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin 300457, China Tianjin Engineering Lab of Efficient and Green Amino Acid Manufacture, Tianjin 300457, China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]代谢控制发酵技术国家地方联合工程实验室,天津300457 [3]天津市氨基酸高效绿色制造工程实验室,天津300457

出　　处：《发酵科技通讯》2016年第4期215-219,共5页Bulletin of Fermentation Science and Technology

摘　　要：谷氨酸棒杆菌(Corynebacterium glutamicum)中ldh基因编码乳酸脱氢酶,可以催化丙酮酸转化生成乳酸,ack A和pqo基因编码乙酸激酶和丙酮酸:醌氧化还原酶,可以催化丙酮酸转化为乙酸。为了研究这三个基因缺失对谷氨酸棒杆菌α-酮戊二酸积累的影响,以C.glutamicum GDK-10为出发菌株,通过重叠延伸PCR及同源重组技术分别构建ldh,pqo,ack A,pqo和ack A基因缺失突变株GDK-14,GDK-15,GDK-16,GDK-17。对出发菌及上述重组菌株进行发酵验证比较,发酵结果表明:ldh和pqo基因缺失菌株的α-酮戊二酸产量分别比出发菌降低了8.54%和27.9%,而ack A基因的缺失使α-酮戊二酸产量比出发菌提高了8.99%。The ldh gene encodes lactic dehydrogenase in Corynebacterium glutamicum, which catalyzes the transformation of pyruvic acid into lactate. The ack A and pqo genes encode acetate kinase and pyruvate quinine oxidoreductase, respectively, which convert pyruvic acid into acetate.In order to investigate the effect of gene deletion on a-ketoglutarate（α-KG） accumulation, the C.glutamicum mutants GDK-14（△ldh）, GDK-15（△pqo）, GDK-16（△ack A）, and GDK-17（△pqo△ack A） were constructed by SOE-PCR and homologous recombination. The fermentation experiments showed that compared to the parental strain CDK-10, the α-KG yield of strain GDK-14 and GDK-16 decreased by 8.54% and 27.9%, respectively, while it reached 47.88 g/L in strain GDK-15 with an increase of 8.99%.

关 键 词：谷氨酸棒杆菌 基因缺失 Α-酮戊二酸 乳酸 乙酸 

分 类 号：TQ922[轻工技术与工程—发酵工程]