萱藻中主要类胡萝卜素的分析与鉴定  

Analysis and identification of major carotenoids from Scytosiphon Lomentaria

作　　者：任丹丹[1] 任先见 王惜童 王丽[1] 汪秋宽[1] 何云海[1] 

机构地区：[1]大连海洋大学食品科学与工程学院,辽宁大连116023

出　　处：《食品工业科技》2017年第2期59-62,68,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金(31301449);辽宁省高校优秀人才项目(LJQ2014077);辽宁省农业领域青年科技创新人才项目(2015005)

摘　　要：采用有机溶剂浸提法从萱藻中提取类胡萝卜素,利用柱层析与薄层层析对萱藻主要类胡萝卜素组分进行了分离纯化。利用可见吸收光谱、红外光谱与高效液相-质谱联用等检测手段对萱藻中主要类胡萝卜素组分进行了分析鉴定,并通过高效液相法对萱藻中类胡萝卜素含量进行了测定。结果表明,萱藻中主要类胡萝卜素为β-胡萝卜素和岩藻黄素,其含量分别为124.55、2862.20μg/g(dw)。Carotenoids from Scytosiphon Lomentaria were extracted by organic solvent method in this paper. Carotenoids were separated and purified by column and thin-layer chromatography. The major carotenoids from Scytosiphon Lomentaria were analyzed and identified by visible spectrum, infrared spectroscopy and HPLC - MS. The contents of major carotenoids from Scytosiphon Lomentaria were determined by HPLC method. The results showed that the major carotenoids from Scytosiphon Lomentaria were β-carotene and fucoxanthin, which contents were 124.55μg/g（dw） and 2862.20 μg/g（dw） ,respectively.

关 键 词：萱藻 类胡萝卜素 结构鉴定 含量 

分 类 号：TS254.2[轻工技术与工程—水产品加工及贮藏工程]