葡萄皮和葡萄酒抗氧化活性与抑制斑马鱼血管生成研究  

Effect of Crude Phenol Extract in Grape Skin and Wine on Angiogenesis Activity of Zebrafish

作　　者：孙海燕[1,2] 王华[3] 徐志民[4] 李华[3] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]陕西理工大学陕西省资源生物重点实验室,汉中723000 [3]西北农林科技大学葡萄酒学院,陕西杨凌712100 [4]路易斯安那州立大学食品科学系,巴吞鲁日70803

出　　处：《农业机械学报》2017年第1期260-266,共7页Transactions of the Chinese Society for Agricultural Machinery

基　　金："十二五"国家科技支撑计划项目(2012BAD31B00);西北农林科技大学2013年试验成果转化项目(XNY2013-64);国家火炬计划项目(2011GH551976)

摘　　要：对2种葡萄皮及其酿造的葡萄酒中粗酚进行提取,测定其体外DPPH清除率和总抗氧化能力,进一步利用转基因斑马鱼对提取物进行血管生成抑制活性检测。提取物处理斑马鱼胚胎22 h后,统计斑马鱼体节间血管的生长情况及提取物对斑马鱼体节间血管的抑制作用。试验结果显示,葡萄酒和葡萄皮均具有一定的体外抗氧化活性,但葡萄酒的DPPH清除率和总抗氧化能力均高于葡萄皮,其中葡萄酒1的DPPH清除率最高,为89.42%;比葡萄酒2高1.29个百分点,总抗氧化能力葡萄酒2最高,为223.24 U/m L;葡萄酒粗酚提取物能明显抑制斑马鱼血管生成,且节间血管数量较对照组明显减少,其中葡萄酒1的背长轴和体节间血管抑制效果更好;而葡萄皮粗酚提取物对斑马鱼血管生成无任何作用。Crude polyphenols were extracted from two types of grape skins and two wines. Total antioxidant activities and in vitro DPPH free radical scavenging activities of the extracts were evaluated.Meanwhile,the effect of those extracts on angiogenesis inhibition was investigated by using transgenic zebrafish model. After zebrafish embryos were treated with the crude polyphenol extracts for 22 h,the inhibitory effect on the intersegmental vessel（ ISV） of zebrafish was investigated. The results indicated that the wines and grape skins all had in vitro antioxidant activities. However,the DPPH free radical scavenging activity and total antioxidant activity of wines were higher than those of grape skins. Wine1 had the highest DPPH free radical scavenging activity（ 89. 42%）,which was 1. 29 percentage points higher than that of wine 2. Wine 2 had the highest total antioxidant activity of 223. 24 U / m L. Compared with control groups,zebrafish treated with crude polyphenol extracts of wines had significantly less ISV formation. Wine 1 had better inhibitory activity on ISV and dorsal longitudinal anastomotic vessel（ DLAV） formation. However,the crude polyphenol extracts of grape skins showed little inhibitory effects on ISV and DLAV formation of zebrafish.

关 键 词：葡萄酒 多酚类物质 抗氧化活性 斑马鱼 血管生成 

分 类 号：TS201.2[轻工技术与工程—食品科学] R730.1[轻工技术与工程—食品科学与工程]