酶法制备南瓜籽壳纤维低聚糖  

Preparation of Cello-oligosaccharides from Pumpkin Seeds Shell Fiber by Enzymatic Method

作　　者：张珍林[1] 

机构地区：[1]皖西学院生物与制药工程学院,安徽六安237012

出　　处：《皖西学院学报》2016年第5期75-78,共4页Journal of West Anhui University

基　　金：六安市定向委托皖西学院市级研究项目(2013LW020)

摘　　要：以南瓜籽壳纤维为原料,采用酶法制备纤维低聚糖,并结合高效液相色谱法(HPLC)进行定量测定。结果表明:利用β-葡聚糖酶在酶解温度50℃、酶解时间2.5h、酶用量4U/g的情况下酶解,可以得到较高比例的葡萄糖、纤维二糖、纤维三糖和纤维四糖,最终计算纤维低聚糖得率为12.2g/100g。An enzymatic method for the preparation of cello-oligosaccharides from pumpkin seeds shell fiber was developed,and HPLC was used for quantitative determination.The results showed that the hydrolysis obtained after 50℃ of hydrolysis with 2.5h of hydrolysis with 4 U/gβ-glucanase contained a high proportion of glucose,cellobiose,cellotriose and cellotetrose,and the yield of cello-oligosaccharides was 12.2g/100 g under these conditions.

关 键 词：南瓜籽壳纤维 纤维低聚糖 Β-葡聚糖酶 

分 类 号：TS241[轻工技术与工程—制糖工程]