裂褶菌发酵转化大豆异黄酮的条件优化  

Optimization of conversion process of soybean isoflavone by Schizophyllum commune

作　　者：刘海英[1] 

机构地区：[1]内蒙古商贸职业学院食品工程系,内蒙古呼和浩特010070

出　　处：《粮食与油脂》2017年第1期79-82,共4页Cereals & Oils

摘　　要：利用裂褶菌发酵β–葡萄糖苷酶将大豆异黄酮糖苷转化为苷元,研究底物浓度、pH、温度和时间对糖苷转化率的影响,采用正交试验优化转化条件。结果显示:最佳条件为底物浓度15g/100mL、温度50℃、转化时间20h,此时测得大豆苷和染料木苷的转化率分别为99.14%和98.57%。The soybean isoflavone was transformed to the free-type aglycone by β-glucosidase from the Schizophyllum commune fermentation.The effect of substrate concentration,pH,temperature and time on conversion rate of hydrolysis and the orthogonal test for optimizing conversion conditions were studied.The results showed that the optimum conversion conditions were substrate concentration 15 g/100 mL,temperature 50 ℃ and time 20 h.Under these conditions,conversion rate of daidzin and genistin was 99.14% and 98.57%,respectively.

关 键 词：裂褶菌 β–葡萄糖苷酶 大豆异黄酮 生物转化 

分 类 号：TS201.3[轻工技术与工程—食品科学]