肺炎支原体对红霉素体外耐药性与耐药基因相关性研究  

Study on the relationship between erythromyc in sensitivity and drug-resistance gene in Mycoplasma pneumoniae

作　　者：刘艳[1] 黄泽智[1] 蒙松年[1] 唐翠连[1] LIU Nan HUANG Zezhi MENG Songnian TANG Cuilian(Department of Medical Laboratory, Shaoyang Medical College, Shaoyang, Hunan 422000, Chin)

机构地区：[1]邵阳医学高等专科学校医学检验系,湖南邵阳422000

出　　处：《国际检验医学杂志》2017年第2期148-150,共3页International Journal of Laboratory Medicine

基　　金：湖南省高等学校科学研究优秀青年基金资助项目(14B164)

摘　　要：目的探讨肺炎支原体(Mp)对红霉素耐药与耐药基因之间的关系。方法通过对46株红霉素耐药Mp临床分离株的23SrRNAⅤ区进行聚合酶链反应(PCR)扩增,并将扩增产物进行基因测序,确定其基因型。结果 46株红霉素耐药Mp临床分离株中有44株(95.65%)发生A2063G突变,2株(4.35%)A2064G突变,未见A2063C和C2617G/A位点突变。结论 Mp临床分离株对红霉素耐药与23SrRNAⅤ区基因2063、2064位点A→G突变有密切关系,其中最主要的突变是A2063G突变。快速、准确识别Mp的23SrRNAⅤ区基因突变可在诊断的同时给出耐药信息,有效指导临床合理用药。Objective To study the relationship between erythromycin sensitivity and drug-resistance gene in Mycoplasma pneumoniae（Mp）.Methods In 46erythromycin-resistant MP clinical isolates,domain Ⅴ of 23 SrRNA was amplified by polymerase chain reaction（PCR）,followed by direct automatic sequencing method.The DNA sequences were compared to find molecular mechanisms of drug resistance.Results Among the 46erythromycin-resistant Mp clinical isolates,44（95.65%）harbored an A-to-G transition mutation at position 2063 in the 23 SrRNA gene and 2（4.35%）harbored an A-to-G transition mutation at position 2064,but no A-to-C transition mutation at position 2063 and C-to-G/A transition mutation at position 2617 were detected.Conclusion Erythromycin-resistant of Mp clinical isolates were closely related to A-to-G transition mutation at position 2063/2064 in domainⅤof 23 SrRNA genes and the most important was the A2063 Gtransition mutation.Rapid and accurate identification of the genetic mutations in domain Ⅴ of 23 SrRNA may be help to diagnose the infection of Mycoplasma pneumoniae,provide the drug-resistant information,and instruct the application of antibiotics reasonably and effectively.

关 键 词：肺炎支原体 红霉素 耐药基因 23S RRNA 点突变 

分 类 号：R725.6[医药卫生—儿科] R440[医药卫生—临床医学]