微生物代谢工程的研究进展和展望  

Progress and prospect in microbial metabolic engineering

作　　者：顾洋[1] 李江华[1] 堵国成[1,2] 陈坚[1,2] 刘龙[1,2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,无锡214122 [2]江南大学糖化学与生物技术教育部重点实验室,无锡214122

出　　处：《生物产业技术》2017年第1期64-70,共7页Biotechnology & Business

基　　金：国家优秀青年基金(31622001);自然基金面上项目(31671845)

摘　　要：代谢工程技术是构建微生物细胞工厂的重要方法,其主要目标是通过基因工程等手段将目标代谢产物产量最大化。然而基因工程等操作往往会影响细胞生长速率,导致其生产强度降低。随着合成生物学及相关技术的发展,多种调控策略被应用于代谢工程领域以解决上述问题。通过这些调控可以有效地解决细胞生长与产物合成之间的竞争关系,平衡代谢途径,避免中间代谢产物的过量积累。对这些策略的研究及应用进行了概述和展望。Metabolic engineering has been proven crucial for the construction of microbial cell factories. The main objective of metabolic engineering is to increase the titer, yield and productivity of target chemicals through genetic engineering. However, genetic manipulations usually result in lower productivity due to growth impairment. With the development of tools in synthetic biology, there has been increasing interest in the dynamic regulation of metabolic flux to overcome the issues. Some metabolic engineering strategies allow trade-offs between growth and production to be better managed and can help avoid build-up of undesired intermediates. This paper summarizes the recent progress in the metabolic engineering and also prospects the future trends in this field.

关 键 词：代谢工程 动态调控 合成生物学 发酵 

分 类 号：Q93[生物学—微生物学]