金线莲乙醇提取物对金黄色葡萄球菌抑菌性能研究  

Antibacterial Activity of the Ethanol Extract of Anoectochilus roxburghii on Staphylococcus aureus

作　　者：黄聪亮[1] 邹兰[2] 胡月英[3] 黎智明 陈文学[2] 

机构地区：[1]漳州职业技术学院,福建漳州363000 [2]海南大学食品学院,海南海口570228 [3]海南大学材料与化工学院,海南海口570228

出　　处：《热带作物学报》2017年第1期150-154,共5页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(No.31640061);海南省自然科学基金(No.312073)

摘　　要：用乙醇作为溶剂,提取出金线莲中的有效抑菌物质,采用二倍稀释法确定金线莲乙醇提取物对金黄色葡萄球菌的最小抑菌浓度(Minimal inhibition concentration,MIC)为5.000 mg/m L;并经介质pH、热处理和紫外线处理提取物,发现其抑菌圈直径在pH9.0和30℃下最大;分析金线莲乙醇提取物对供试菌蛋白质和还原糖的利用能力,表明金线莲乙醇提取物通过减弱蛋白质和还原糖的代谢来抑制细菌的生长繁殖。Anoectochilus roxburghii was extracted by ethanol. The minimal inhibition concertation （MIC） was detected by the broth two-fold agar dilution method and Staphylococcus aureus MIC was 5 mg/mL. Besides, the inhibitory zone diameters of the ethanol extract on A. roxburghii was up to the maximum at pH9 and 30 ℃, respectively. The protein and sugar concentrations in the bacterium were determined, and the results showed that the ethanol extract of A. roxburghii could inhibit the metabolism of the two substances, which affected the growing of the bacterium.

关 键 词：金线莲 抑菌机理 最小抑菌浓度 稳定性 

分 类 号：R284.2[医药卫生—中药学]