银耳菌糠黑色素微波辅助提取工艺优化及其抗氧化活性  

Study on extraction process and antioxidant activity of melanin from Tremella fuciformis residue

作　　者：师萱[1] 阎语 梁旭明[1] 谭红军[1] 杨勇[1] 钟耕[2] 石文娟 

机构地区：[1]重庆市中药研究院,重庆400065 [2]西南大学食品科学学院,重庆400716 [3]银耳营养食品企业工程技术研究中心,重庆409000

出　　处：《食品与机械》2016年第12期161-165,174,共6页Food and Machinery

基　　金：重庆市基本科研业务费计划项目(编号:2014cstc-jbky-01902)

摘　　要：以微波辅助碱溶酸沉法提取银耳菌糠的天然黑色素,优化其分离纯化工艺,并对黑色素的光谱特征和抗氧化活性进行研究。结果表明,微波辅助碱溶酸沉法提取银耳菌糠黑色素最佳工艺为:料液比140(g/mL),提取碱液浓度1.75mol/L,水解时间2h,微波处理时间60s,该条件下黑色素粗提物的色价为156.6,经纯化后色价达316.0。经红外光谱分析,将银耳菌糠黑色素归为3,4-二羟基苯丙氨酸(DOPA)类的黑色素。其对DPPH自由基的半抑制浓度为1.74mg/mL,O_2^-自由基的半抑制浓度为1.08mg/mL。Melanin was extracted by alkali solution and acid extraction method with the assistance of microwave from Tremella fuciformis residue.The separation and purification was optimized.The spectral characteristics and antioxidant activities of melanin were researched.The results showed that the optimum process of extracting melanin from Tremella fuciformis residue by alkali solution and acid extraction method with the assistance of microwave were as follows：amount of extraction solvent to yield 140,concentration of NaOH1.75mol/L,hydrolyzing time 2h,microwave treatment time 60 s.In this condition color value of melanin extracted fromTremella fuciformis residue was 156.6,and was 316.0after purification.By spectroscopic,melanin from Tremella fuciformis residue was identified to be DOPA.The half clearance rates of DPPH radical and ·O_2^-radical were 1.74,1.08mg/mL,respectively.

关 键 词：银耳菌糠 黑色素 微波 分离纯化 光谱特性 抗氧化活性 

分 类 号：TS209[轻工技术与工程—食品科学]