努格特啤酒花挥发性成分的气相色谱-质谱分析  

Analysis of the volatile compounds from Nugget hop variety by gas chromatography-mass spectrometry

作　　者：李帅[1] 刘玉梅[1] LI Shuai LIU Yu-mei(College of Chemistry ＆ Chemical Engineering,Xinjiang university, Urumqi 830046, Chin)

机构地区：[1]新疆大学化学化工学院,新疆乌鲁木齐830046

出　　处：《食品工业科技》2017年第3期279-285,289,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(31360403)

摘　　要：采用气相色谱-质谱联用(Gas chromatography-Mass spectrometer,GC-MS)方法,谱库检索结合保留指数(retention index,RI)定性,比较了顶空固相微萃取(Headspace-solid phase microextraction,HS-SPME)和水蒸气蒸馏法(Steam distillation,SD)得到的国产努格特啤酒花的挥发性成分,同时采用峰面积归一化法对各成分进行定量,并与美国原产地该酒花的主要挥发性风味成分进行对照。结果表明,两种方法检测的挥发性成分存在较大差异。HS-SPME法定性了106个挥发性成分,相对含量为99.11%,而SD法仅定性88个成分,相对含量为94.38%。努格特啤酒花的挥发性成分以碳氢化合物(包括萜烯类化合物)为主,其次为酯类化合物和醇类化合物;含有的主要风味成分有β-蒎烯、β-香叶烯、反式-石竹烯、α-葎草烯和L-芳樟醇等,且上述指标均与美国原产地该啤酒花品种的主要挥发性风味成分含量范围一致或接近,表明引种后的努格特啤酒花基本上保持了原产地品种的风味成分。Head space- solid phase microextraction- gas chromatography- mass spectrometry （ HS- SPME- GC- MS） and steam distillation-gas chromatography-mass spectrometry（SD-GC-MS） were compared to analyze the volatile compounds of Nugget hop varirty grown in China. The qualitative and quantitative analysis were performed using mass spectral library retrieval combined retention index（RI） and the normalization method of peak area, respectively. Meanwhile, the content of main flavor compounds were compared with the hop grown in the USA.It was shown that the significant difference was obtained of volatile compounds of Nugget bop by HS-SPME and SD.A total of 106 volatile compounds was identified by HS-SPME, and accounted for 99.11% of the total peak area.While only total of 87 compounds was obtained by SD, accounted for 96.20% .The results showed that the main volatile composition was hydrocarbon compounds （ include terpene compounds）, followed by esters and alcohols.The main volatile flavor compounds were β- pinene ,β- myrcene, trans- caryophyllene, α- humulene, L- linalool, etc., which were agreed or closed to the major volatile compounds of Nugget hop variety grown in the USA.It indicated that the flavor compounds were complete in accordance with the Nugget hop variety introducted from foreign and their origin.

关 键 词：努格特啤酒花 挥发性成分 顶空固相微萃取(HS—SPME) 水蒸气蒸馏(SD) 气相色谱-质谱联用(GC—MS) 

分 类 号：TS261.2[轻工技术与工程—发酵工程]