HS-SPME-GC-MS分析炮制前后苍耳子挥发性成分  

Analysis of Volatile Compositions in Xanthium Sibiricum Patr. Before and After Processing by HS-SPME-GC-MS

作　　者：周涛[1] 邱红汉[1] Zhou Tao Qiu Honghan(Wuhan Hospital of Traditional Chinese Medicine, Wuhan 430014, China)

机构地区：[1]武汉市中医医院,武汉430014

出　　处：《中国药师》2017年第2期235-237,241,共4页China Pharmacist

摘　　要：目的:分析苍耳子生品及炒制品中挥发性成分的化学成分及相对含量的变化。方法:采用顶空固相微萃取法(HSSPME)结合气相色谱-质谱(GC-MS)联用技术对苍耳子的生品及炒制品挥发性成分进行分析;以峰面积归一化法计算各组分的相对百分含量。结果:从苍耳子生品和炒制品中分别分离鉴定出26种和27种化合物,共有成分19种,分别占挥发性成分总量的68.69%和90.08%,其中生品含量较高的成分有桉叶油醇(15.3%)、樟脑(9.6%)、冰片(6.89%)等,炒制品中成分含量较高的成分有薄荷醇(24.09%)、冰片(18.48%)、桉叶油醇(7.19%)等。结论:炒制品中薄荷醇、冰片等具有治疗鼻炎作用的相对含量较生品有明显提高,采用HS-SPME-GC-MS联用技术分析苍耳子生品及炒制品中挥发性成分及相对含量变化,可以从挥发性成分的变化方面为苍耳子生品及炒制品的功效差异的物质基础奠定一定的理论基础,为其进一步研究和综合利用提供科学依据。Objective ： To analyze the changes of chemical compositions and relative contents of volatile constituents in raw Xanthi- um sibiricum Patr. and fried Xanthium sibiricum Patr. Methods ： Headspace solid phase micro extraction （HS-SPME） combined with gas chromatography-mass spectrometry （GC-MS） was used to study the volatile components in raw and fried products of Xanthium sibir- icum Patr： A peak area normalization method was applied to calculate the relative percentage content of each composition. Results： Totally 26 and 27 kinds of compounds were isolated and identified respectively from raw and fried xanthium including 19 species and re- spectively accounting for 68.69% and 90.08% of the total volatile components. In the raw products, the components with high con- tents were camphor （15.3%）, eucalyptus oil alcohol （9.6%） and borneol （6.89%）, while in the fried products, those were men- thol （24.09%） , borneol （18.48%） and eucalyptol （7.19%）. Conclusion： The relative contents of components with effects on rhi- nitis in the fried products are obviously higher than those in the raw products. HS-SPME-GC-MS can be used to detect the volatile com- positions and their relative contents in the raw products and fried products of Xanthium sibiricum Parr.. From the changes of volatile components, theoretical basis can be laid for the components with certain efficacy in raw and fried Xanthium sibiricum Patr. , and scien- tific basis can be provided for their further study and comprehensive utilization.

关 键 词：苍耳子生品及炒制品 顶空-固相微萃取 气相色谱-质谱联用 挥发性成分 

分 类 号：R284.1[医药卫生—中药学]