胶红酵母菌降解毒死蜱发酵工艺研究  

Optimization Fermentation of Rhodotorula mucilaginosa for Chlorpyrifos Degradation

作　　者：梁金钟[1] 梅剑秋 王翼雪 

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江省高校食品科学与工程重点实验室,黑龙江哈尔滨150076

出　　处：《农产品加工》2017年第1期45-50,54,共7页Farm Products Processing

摘　　要：为提高胶红酵母菌RM-DY21对有机磷农药的降解率,以YPD培养基为基础培养基进行碳源、氮源及培养条件优化。利用单因素和Box-Behnken响应曲面分析,确定了胶红酵母菌RM-DY21优化培养基配方为乳糖15 g/L,硫酸铵25 g/L,酵母浸粉10 g/L;胶红酵母菌RM-DY21降解有机磷农药的最佳培养条件为初始pH值5,培养温度30℃,培养时间36 h。利用优化后的发酵条件进行培养,结果显示胶红酵母菌RM-DY21对有机磷农药的降解率达到83.86%,比优化前提高了10.62%。Rhodotorula mucilaginosa are cultured for the degradation of organic phosphorus pesticide. To improve the degradation rate of organic phosphorus, carbon source, nitrogen source and fermentation conditions are optimized in YPD culture medium. With the results of single factor and Box- Behnken analysis, the optimum culture medium is as follows：lactose 15 g/L,ammonium sulfate 25 g/L,yeast extract 10 g/L and optimal culture conditions are as follows：initial pH 5,fermentation temperature 30 ℃,fermentation time 36 h. In the optimized medium,organic phosphorus pesticide degradation rate of Rhodotorula mucilaginosa reached to 83.86% which is 10.62% higher than that without optimization.

关 键 词：胶红酵母菌 培养基 发酵条件 优化 

分 类 号：TQ920[轻工技术与工程—发酵工程]