表面等离子共振技术快速检测食品中的玉米赤霉烯酮毒素  

Rapid detection of zearalenone in food by surface plasmon resonance technology

作　　者：赵芳[1] 涂晓波[1] 黄欣迪 张恒[1] 洪小柳[1] 刘慧玲[1] 吕敬章[1] 

机构地区：[1]深圳出入境检验检疫局食品检验检疫技术中心,深圳518045

出　　处：《食品安全质量检测学报》2016年第12期4849-4852,共4页Journal of Food Safety and Quality

基　　金：国家质量监督检验检疫总局科技计划项目(2015IK251;2015IK252);深圳出入境检验检疫局科技项目(SZ2015207;SJ201403)~~

摘　　要：目的建立基于表面等离子共振(surface plasmon resonance,SPR)技术快速检测食品中玉米赤霉烯酮毒素(zearalenone,ZEN)的方法。方法采用表面自组装技术(self-assembled monolayer,SAM)在金膜的表面修饰羧基基团,将ZEN抗原与牛血清白蛋白(albumin from bovine serum,BSA)偶联物(ZEN-BSA)通过共价键固定在芯片的表面,采用竞争法检测样品中的玉米赤霉烯酮毒素。结果该方法的检测限为8.2 ng/m L,ZEN单克隆抗体与呕吐毒素、黄曲霉毒素B_1、赭曲霉毒素、伏马毒素等没有交叉反应,与α-玉米赤霉烯醇和β-玉米赤霉烯醇交叉反应率分别为15.3%和11.5%。结论本方法具有简便、快速和高灵敏度等优势,在食品中真菌毒素的快速检测方面具有潜在的应用价值。Objective To establish a method for the rapid detection of zearalenone （ZEN） by surface plasmon resonance （SPR） technology.Methods The surface of the gold film was modified with carboxy group by self-assembled monolayer （SAM）. The ZEN-BSA conjugate was immobilized on SPR chip through covalent bond, and the competition method was used to detect ZEN in food.Results The detection limit of this method was 8.2 ng/mL. The ZEN monoclonal antibody did not show cross reaction with deoxynivalenol, aflatoxin B1, ochratoxin and fumonisin, and the cross reaction rates withα-zearalenol andβ-zearalenol were respectively 15.3% and 11.5%. Conclusion The established method is simple, rapid, highly sensitive, which has the potential application value in the rapid detection of mycotoxins in food.

关 键 词：玉米赤霉烯酮 表面等离子共振 酶联免疫法 真菌毒素 

分 类 号：TS207.3[轻工技术与工程—食品科学]