应用多重实时荧光PCR方法确诊一起副溶血性弧菌所致食物中毒  

Detection of a Vibrio parahaemolyticus caused outbreak of food poisoning by multiplexed real-time fluorescence PCR

作　　者：谭冬梅[1] 王红[1] 吕素玲[1] 李秀桂[1] 曾献莹[1] 苏爱荣[1] 

机构地区：[1]广西壮族自治区疾病预防控制中心,广西南宁530028

出　　处：《中国卫生检验杂志》2017年第3期344-346,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的采用多重实时荧光PCR对一起疑似食物中毒的样本进行快速检测,结合病原学的结果,确定病原体。方法2015年5月广西东兰县发生了一起疑似细菌性食物中毒。应用多重实时荧光PCR方法对留存食品和患者肛拭子标本进行副溶血性弧菌4种毒力基因的检测,同时对样本进行病原学检测。结果共检测15份食品样本和12份肛拭子标本,除了从1份肛拭子标本中分离培养出O3∶K6副溶血性弧菌外,其余标本均未分离出食源性致病菌。从8份肛拭子标本中检出副溶血性弧菌毒力基因,其中所有菌株均携带tdh、tlh和orf8基因,3株携带trh基因。结论这是广西首次利用多重实时荧光PCR技术确诊由副溶血性弧菌引起的食物中毒。多重实时荧光PCR方法可以同时鉴定和检测副溶血性弧菌及其毒力基因,具有快速、灵敏、准确的优点,可为食源性暴发事件提供快速、可靠的检测结果。Objective To determine the pathogen for an outbreak of food poisoning by multiplexed real- time polymerase chain reaction（ PCR） assay,and to determine pathogen according to pathogenic results. Methods An outbreak of suspected bacterial food- borne gastroenteritis occurred in Donglan,Guangxi,in May,2015. Food and rectal swabs samples were tested by multiplexed real- time PCR assay for the detection of 4 virulence- associated genes of V. parahaemolyticus. Meanwhile,these samples were conducted for pathogenic detection. Results A total of 15 food samples and 12 rectal swabs were tested. No bacterial pathogens were detected except one isolate of V. parahaemolyticus from a rectal swab sample. Virulence genes were detected from 8 of the samples,with all containing tdh,tlh and orf8,and 3 containing trh. Conclusion This is the first report in Guangxi to confirm V. parahaemolyticus caused food poisoning using multiplexed real- time fluorescent PCR assay. The assay,which is able to detect both the presence of V. parahaemolyticus and virulence genes,is advantageous on rapidity,high sensitivity and accuracy,and may provide rapid and reliable results for food- borne outbreaks.

关 键 词：多重实时荧光PCR 副溶血性弧菌 食物中毒 毒力基因 

分 类 号：R155[医药卫生—营养与食品卫生学]