响应面法优化鼠李糖乳杆菌产共轭亚油酸的转化条件  

Optimization of Transformation Conditions for Producing Conjugated Linoleic Acid in Lactobacillus rhamnosus by Response Surface Method

作　　者：方丽[1] 张芷睿 王鹏[1] 王祖浩[1] 闵伟红[1] FANG Li ZHANG Zhirui WANG Peng WANG Zuhao MIN Weihong(College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China)

机构地区：[1]吉林农业大学食品科学与工程学院,长春130118

出　　处：《吉林农业大学学报》2016年第6期754-758,765,共6页Journal of Jilin Agricultural University

基　　金：吉林省教育厅"十二五"科学技术研究项目[吉教科合字(2012)第57号]

摘　　要：以鼠李糖乳杆菌UV3-4为出发菌株,进行鼠李糖乳杆菌转化亚油酸生产共轭亚油酸转化条件的优化研究。通过单因素试验考察发酵温度、发酵时间、发酵pH和底物亚油酸(LA)添加量对CLA产量的影响,采用响应面法建立CLA产量与各影响因子之间的二次回归方程模型,获得最佳转化条件:发酵温度38℃,发酵时间23.4 h,pH 6.5,底物LA添加量为0.76 mg/m L,此条件下CLA的产量为37.28μg/m L。This study aims at conditions optimization of the transformation of linoleic acid to conjuga- ted linoleic acid by Lactobacillus rhamnosus UV3-4. The effects of fermentation temperature, fer- mentation time, fermentation pH and LA concentration on the yield of CLA were investigated by sin- gle factor test. Response surface method was used to establish the model of the two regression equa- tions between CLA yield and influencing factors. The optimum conditions were as follows： fermenta- tion temperature 38 ℃, fermentation time 23.4 h, pH 6.5, and addition amount LA 0. 76 mg/mL. Under the above conditions, the yield of CLA was 37.28 μg/mL.

关 键 词：共轭亚油酸 鼠李糖乳杆菌 响应面法 转化条件 

分 类 号：Q78[生物学—分子生物学]