降胆固醇双歧杆菌BZ11的筛选及其耐受性评价  

Screening and tolerance evaluation of cholesterol-lowering Bifidobacterium BZ11

作　　者：朱啟会 何腊平[1,2] 张玲[1] 高冰[3] 李翠芹[4] 熊江[1] 

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州省农畜产品贮藏与加工重点实验室,贵州贵阳550025 [3]湖北工业大学生物工程与食品学院,湖北武汉430068 [4]贵州大学化学与化工学院,贵州贵阳550025

出　　处：《中国酿造》2017年第3期54-58,共5页China Brewing

基　　金：国家自然科学基金(31160002);黔科合重大专项字[2015]6004-5号

摘　　要：采用平板涂布法从贵州特色香猪粪便中筛选具有降胆固醇能力及耐酸、耐胆盐和耐氧性能的双歧杆菌(Bifidobacterium)。从香猪粪便中共筛选出19株具有双歧杆菌形态特征的菌株。采用邻苯二甲醛法测定其降胆固醇率,得到7株降胆固醇能力较高菌株,其中菌株BZ11降胆固醇率最高可达(38.52±0.60)%,并对酸、胆盐和氧具有较高的耐受性。经菌落形态特征、生理生化试验及16S r RNA序列分析,菌株BZ11被鉴定为动物双歧杆菌乳亚种(Bifidobacterium animalis subsp.lactis)。Bifidobacterium with the ability of cholesterol-lowering and the tolerance of acid, cholate and oxygen was screened from Guizhou Xiang pig faeces by spread-plate method. A total of 19 strains were screened. The cholesterol-lowering rate of strains was detected by phthalic aldehyde method, and 7 strains with high cholesterol-lowering ability were obtained. The cholesterol-lowering rate of strain BZ11 was up to （38.52±0.60）%, and the strain had higher tolerance of acid, cholate and oxygen. By colonial morphology, physiological biochemical experiments and 16S rRNA sequence analysis, strain BZ11 was identified as Bitidobacterium animalis subsp, lactis.

关 键 词：双歧杆菌BZ11 降胆固醇 筛选 鉴定 耐受性 

分 类 号：TS261.1[轻工技术与工程—发酵工程]