粗糙脉孢菌无性产孢过程中增量表达基因的功能分析  

Functional analysis of up-regulated genes during Neurospora crassa conidiation

作　　者：王飞[1,2] 胡成成[2] 刘波[1] 李少杰[2] 孙宪昀[2] 

机构地区：[1]齐鲁工业大学食品科学与工程学院,山东济南250353 [2]中国科学院微生物研究所真菌学国家重点实验室,北京100101

出　　处：《菌物学报》2017年第3期311-322,共12页Mycosystema

基　　金：国家自然科学基金(31370024)~~

摘　　要：无性产孢是丝状真菌主要的繁殖方式,也是病原真菌传播的基础。为了全面分析丝状真菌无性产孢的调控机制,我们以粗糙脉孢菌为模式菌株,利用RNA‐seq比较了诱导无性产孢前后的转录组变化。对诱导前后差异表达基因的聚类分析发现,无性产孢诱导主要影响氧化还原过程与代谢过程,与ROS(reactive oxygen species)相关的基因差异表达明显,无性产孢诱导阶段伴随ROS的升高。同时,与产孢相关的基因(包括调控基因和结构基因)出现特异性表达。为揭示其他诱导表达基因在无性产孢中的功能,我们对这些基因缺失突变体的无性产孢表型进行了分析,新发现6个正向影响无性产孢的基因[NCU09792、NCU05159、NCU06112、NCU05079、NCU00461、NCU07521(fwd‐2)]。这6个基因在无性产孢过程中增量表达,相应的基因突变体无性产孢量与野生菌株相比有明显降低,说明它们对无性产孢具有正调控作用。以上结果进一步加深了我们对粗糙脉孢菌无性产孢发育及其调控网络的认识。Conidiation is a biological process important for filamentous fungi to reproduce, disperse and ultimately form infection n order to elucidate the regulatory mechanisms of conidiation, transcriptomic profiles generated by RNA-seq from the samples of the model fungus Neurosporo crassa wild-type strain before and after induction of conidiation were compared. Gene ontology analysis of differentially expressed genes indicated that oxidation-reduction process and metabolic process are mainly nfluenced during the induction of conidiation. Among these, genes related to reactive oxygen species （ROS） are enriched, ndicating the ROS production during conidiation. Moreover, genes associated with conidiation （including regulatory genes and spore structural genes） expressed specifically. To understand the roles of these induced genes in conidiation, we analyzed the phenotype of the mutants for 424 up-regulated genes and found six genes [NCU09792, NCU05159, NCU06112, NCU05079, NCU00461, NCU07521 ~fwd-2）] that positively contribute to conidiation. The conidial productions of these gene deletion mutants are much lower than that of the wild type strain, and the increased expression of these genes in conidiations indicates that they are involved in positive regulation of conidiation. Our work facilitates the understanding of conidiation and its regulatory networks in N. crassa

关 键 词：粗糙脉孢菌 无性产孢 转录表达谱 活性氧簇(ROS) 

分 类 号：Q949.32[生物学—植物学]