响应面法优化新疆奶花芸豆α-淀粉酶抑制剂的提取工艺  

Optimization of extraction of alpha-amylase inhibitor from Phaseolus vulgaris by response surface methodology

作　　者：于雪慧 田童童 张建[1] YU Xue-hui TIAN Tong-tong ZHANG Jian(Food College of Shihezi University,Shihezi 832003, Chin)

机构地区：[1]石河子大学食品学院,新疆石河子832003

出　　处：《食品工业科技》2017年第6期223-228,共6页Science and Technology of Food Industry

摘　　要：利用响应面法研究新疆奶花芸豆中α-淀粉酶(α-AI)抑制剂的提取工艺。在单因素实验的基础上,选取浸提液体积、p H、浸提时间和浸提温度作为考察因素,以α-AI抑制率作为评价指标,利用Box-Behnken中心组合方法设计四因素三水平响应面分析法,建立数学模型,从而确定最佳提取工艺为:准确称取5 g样品,添加浸提液体积200 m L,在p H3.5、43℃下浸提2.3 h,此条件下,α-AI的抑制率达到85.30%,与理论预测值(87.82%)基本相符。结果表明,优化得到的提取工艺稳定可行,为进一步研究和开发利用奶花芸豆奠定了基础。Response surface methodology was applied to optimize the extraction conditions of alpha-amylase inhibitor from Phaseolus vulgaris.Based on the results of the single factor experiments, the effects of four independent variables （volume of extraction solvent, extraction pH value, extraction time and extraction temperature ）on the α- AI inhibition rate were investigated by Box-Behnken experimental design of four factors and three levels.A quadratic polynomial regression equation of the forecasting model was set up.And the optimal extraction parameters to obtain the highest inhibition rate were as follows ：the solvent volume was 200 mL, pH3.5,43 ℃ and 2.3 h for extraction per time.The inhibition rate under the optimum conditions was found to be 85.30%, which agreed with the predicted value of 87.82%. Results showed that the optimized method was stable and reliable.It could lay the foundation for the research, development of the Phaseolus vulgaris.

关 键 词：奶花芸豆 Α-淀粉酶抑制剂 响应面分析 

分 类 号：TS239[轻工技术与工程—粮食、油脂及植物蛋白工程]