气相色谱串联质谱检测豆芽中生长调节剂残留  

Determination of Growth Regulators in Bean Sprout by Gas Chromatography-tandem Mass Spectrometry

作　　者：段丹丹[1] 马青青[2] 张利锋[2] 高强[1] 张榕杰[2] 吕全军[1] 

机构地区：[1]郑州大学公共卫生学院,郑州450001 [2]河南省疾病预防控制中心公共卫生研究所,郑州450016

出　　处：《食品工业》2017年第3期265-268,共4页The Food Industry

基　　金：河南省医学科技攻关项目资助课题(201203060)

摘　　要：建立同时检测豆芽中5种植物生长调节剂残留的固相萃取-气相色谱串联质谱方法。匀浆处理的豆芽试样,用体积分数为0.5%的甲酸乙腈溶液超声提取,经MCS小柱纯化,以HP-5 MS毛细管色谱柱分离,在选择离子监测模式下进行分析,外标法定量。方法可以在26 min内同时检测豆芽中5种植物生长调节剂残留,检出限为3.0~8.0μg/kg,定量限为10.0~25.0μg/kg,相关系数r>0.993,平均加标回收率达到81.4%~101.2%,相对标准偏差为2.4%~7.8%。方法快速、高效、灵敏度高,可用于食品中多种植物生长调节剂残留的同时测定。It was to set up a gas chromatography-tandem mass spectrometric（LC-MS/MS） method with solid phase extraction for the determination of 5 plant growth regulators（PGRs） residues simultaneous. Homogenatized samples were initially extracted with acetonitrile containing 0.5% acetic acid via ultrasonic and cleaned-up by using MCS cartridge, then separated by gas chromatography with capillary column（HP-5MS, 30 m×0.25 mm, 0.25 μm） and determined by a mass spectrometer with electron impact（EI） at selected ion monitoring（SIM） mode. The content was quantified by external standard method. This method could simultaneously detect 5 kinds of plant growth regulators within 26 min. The limits of detection（LOD） were 3.0-8.0 μg/kg and the limits of quantification（LOQ） were 10.0-25.0 μg/kg, the correlation coefficient was greater than 0.993, the average recoveries were 81.4%-101.2% and the relative standard deviations were 2.4%-7.8%. This method is rapid, efficient, and sensitive. It can be used for the determination of multiple plant growth regulators residues in food simultaneously.

关 键 词：植物生长调节剂 豆芽 固相萃取 气相色谱-质谱法 

分 类 号：S481.8[农业科学—农药学] O657.63[农业科学—植物保护]