杏鲍菇与白灵菇红斑病病原菌拮抗菌的筛选与鉴定  

Isolation and Identification of Antagonistic Strains Against Red spot Disease of Pleurotus eryngii and Pleurotus nebrodensis

作　　者：荣成博 胡杰[1,2] 杨永利[1,2] 赵爽[1,2] 王守现[1,2] 马康[1,2] 黄晨阳[3] 刘宇[1,2] 

机构地区：[1]北京市农林科学院植物保护环境保护研究所北京市食用菌工程技术研究中心,北京100097 [2]农业部都市农业(北方)重点实验室,北京100097 [3]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《中国食用菌》2017年第2期56-59,共4页Edible Fungi of China

基　　金：国家科技支撑计划课题食用菌生产质量安全控制关键技术研究(2013BAD16B03);北京市优秀人才培养资助计划(2015000020060G138);北京市农林科学院青年基金项目(QNJJ201524)

摘　　要：筛选并鉴定杏鲍菇和白灵菇红斑病病原菌的拮抗菌,为该病的生物防治提供实验证据。通过牛津杯法筛选拮抗菌,并通过16s r DNA分析进行拮抗菌鉴定。结果表明,筛选到杏鲍菇红斑病病原菌的拮抗菌1株,为GL110,白灵菇红斑病病原菌的拮抗菌2株,分别为GL110和GL52。GL110鉴定为类芽孢杆菌属细菌,GL52鉴定为贪铜属细菌。Isolation and identification of antagonistic strains against red spot disease of Pleurotus eryngii and Pleurotus nebro- densis could provided the experimental evidence for biological control of the disease. The antagonistic strains against red spot disease of P. eryngii and P. nebrodensis were isolated and identified by oxford cup tests and the analysis on 16s rDNA. The re- sults showed that a strain GL110 with inhibitory activity against P. eryngii pathogen, and the strains GL110 and GL52 displayed antagonistic activities against P. nebrodensis pathogen. GL110 and GL52 were identified as Cupriavidus respectively.

关 键 词：杏鲍菇 白灵菇 红斑病 拮抗菌 

分 类 号：S436.46[农业科学—农业昆虫与害虫防治]