新疆产核桃仁挥发性芳香物质成分萃取条件的优化及GC-MS分析  

The Optimization of SPME Condition for Extracting Volatile Aromatic Components of Xinjiang Juglandis Semen Based on GC-MS Analysis

作　　者：安冉[1] 华震宇[1] 王建梅[1] AN Ran HUA Zhen-yu WANG Jian-mei(Institute of Quality Standards ＆Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences, Laboratory of Quali- ty and Safety Risk Assessment for Agro-Products （ Urumqi）, Key Laboratory of Agro-products Quality and Safety of Xinjiang, Urumqi 830091, China)

机构地区：[1]新疆农业科学院农业质量标准与检测技术研究所/农业部农产品质量安全风险评估实验室(乌鲁木齐)/新疆农产品质量安全重点实验室,新疆乌鲁木齐830091

出　　处：《中药材》2016年第10期2295-2299,共5页Journal of Chinese Medicinal Materials

基　　金：新疆农业科学院青年基金项目(xjnkq-2015017);新疆维吾尔自治区科技成果转化专项资金项目(201454122);新疆维吾尔自治区公益性科研院所基本科研业务经费资助项目(KYGY2016110)

摘　　要：目的:优选核桃仁挥发性芳香物质成分的固相微萃取条件,并利用GC-MS法对比分析新疆产新鲜核桃仁与酸败核桃仁样品的芳香物质成分。方法:采用单因素试验和正交试验设计优化固相微萃取条件,利用GC-MS分析对比新鲜核桃仁与酸败核桃仁的成分。结果:优选得到的固相微萃取条件为:采用75μm CAR/PDMS萃取头,取样量为2.0 g,萃取温度为60℃,萃取时间为30 min。分别从新鲜核桃仁和酸败核桃仁中分离鉴定了28种和33种挥发性成分。结论:该实验优选的萃取方法简单、可行,可用于核桃仁中挥发性芳香物质的萃取。Objective： To optimize the solid-phase micro-extraction method（ SPME） condition in extracting the preferred volatile aromatic components from Juglandis Semen,and to analyze the aromatic components of fresh Juglandis Semen and rancid Juglandis Semen by GC-MS method. Methods： Single-factor and orthogonal tests was applied to optimize the condition of SPME,and compared the components of Fresh Juglandis Semen and rancid Juglandis Semen by using GC-MS method. Results： The optimal SPEM condition was as follows,75 μm CAR / PDMS was used as extraction fiber,the sample quantity was 2. 0 g,the extraction temperature was 60 ℃,and extracted for 30 min. 28 and 32 volatile components were extracted and identified from fresh Juglandis Semen and rancid Juglandis Semen,respectively. Conclusion： This method is proved to be simple and feasible,and can be used to extract volatile aromatic components in Juglandis Semen.

关 键 词：核桃仁 固相微萃取 GC-MS 酸败 

分 类 号：R283.6[医药卫生—中药学]