蒸汽爆破对油菜秸秆降解产物的影响  

The Effect of Degradation Products of Rapeseed Straw by Steam Explosion Pretreatment

作　　者：张春艳[1] 谭兴和[1] 熊兴耀[2,3] 苏小军[1,2] 李清明[1] 

机构地区：[1]湖南农业大学食品科技学院,湖南长沙410128 [2]湖南省作物种质创新与资源利用重点实验室,湖南长沙410128 [3]中国农业科学院蔬菜与花卉研究所,北京100081

出　　处：《激光生物学报》2017年第1期78-84,共7页Acta Laser Biology Sinica

基　　金：国家"863计划"项目(2012AA101804);湖南省科技厅项目(2015NK3009)

摘　　要：采用DNS法、离子色谱法和气质联用(GC-MS)技术对油菜秸秆蒸汽爆破降解产物进行了分析。结果表明,经蒸汽爆破处理后的油菜秸秆酶解产糖量有明显的提高,为未处理样品直接酶解含量的3.4倍,各种水溶性糖化合物的含量也大大提高,其中葡萄糖和木糖含量分别为未处理样品直接酶解含量的3.5倍和4.7倍之多。水提液乙酸乙酯萃取物中分别检测到脂肪酸类、芳香类和呋喃类物质40、12和1种。通过扫描电镜观察处理后油菜秸秆的表面形貌结构,发现处理后的秸秆表面结构松散,比表面积增大,酶对纤维素的可及性增加。The degradation products from steam exploded rapeseed straw were investigated by DNS, high-performance anion exchange chromatography （ HPAEC） and GC-MS. The results suggested that the total reducing sugar production af-ter enzymatic hydrolysis enhanced obviously, and it is 3.4 times that of the untreated sample digested by enzyme direct-ly. The content of water-soluble sugar compounds increased greatly were detected by HPAEC, the glucose and xylose content is respectively 3. 5 times and 4. 7 times as much as that of untreated samples digested by enzyme directly. By a-dopting GC-MS, the number of fatty acids, aromatics and furans were 40, 12 and 1 , respectively, which were detected from ethyl acetate fraction in water extract of steam exploded straw. The surface morphology structure of steam exploded rapeseed straw was investigated by SEM, the surface structure became looser after pretreatment, and the enzyme adsorp-tion area increased.

关 键 词：油菜秸秆 蒸汽爆破 降解产物 气质联用 

分 类 号：S216.2[农业科学—农业机械化工程]