薛凡尼氏炭角菌发酵菌丝体提取物的抗氧化活性  

Antioxidant activities of different solvents extracts from mycelium of Xylaria schweinitzii in submerged culture

作　　者：田强[1] 王君[1] 张梦娜[1] 马林[1,2] 贺新生[1,2] 袁小红[1,2] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]四川省生物质资源利用与改性工程技术研究中心,四川绵阳621010

出　　处：《食品工业科技》2017年第7期49-53,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(21272189);国家大学生创新创业训练计划(201410619002)

摘　　要：分别采用4种不同溶剂(乙醇、甲醇、丙酮和水)对薛凡尼氏炭角菌菌丝体进行提取,测定不同溶剂提取物的总酚和总黄酮含量以及对DPPH自由基清除能力和总还原力。结果表明,不同溶剂提取物均具有不同程度的抗氧化活性。在各提取物浓度为2.5 mg/m L时,对DPPH自由基的清除率以水提物为最高(91.8%),其次为60%丙酮提取物和70%乙醇提取物,清除率分别为89.6%和83.6%,甲醇提取物的清除率最低(75.1%)。各溶剂提取物的还原能力强弱依次为:水>60%乙醇>70%乙醇>60%丙酮>甲醇。菌丝体水提物的总酚含量最高,而总黄酮含量最高的是甲醇提取物。总体而言,薛凡尼氏炭角菌菌丝体水提物具有较强的抗氧化活性,可能具有开发为食品抗氧化剂的潜力。Four different solvents （ ethanol, methanol, acetone and water ） were used for extracting the mycelium of Xylaria schweinitzii.The content of total phenolics and total flavonoids in the extracts from different solvents was analyzed and the scavenging capacity of DPPH radical scavenging capacity and total reducing power were determined.The results showed that the different solvent extracts had different antioxidant activity. At the concentration of each extract 2.5 mg,/mL, the water extract showed the highest radical scavenging rate（91.8% ）on DPPH,followed by 60% acetone extract and 70% ethanol extract, the scavenging rates were 89.6% and 83.6% ,and the methanol extract showed the lowest scavenging rate which was （75.0%）on DPPH.The reduction ability of the different solvent extracts was in the order： water 〉 60% ethanol 〉 70% ethanol 〉 60% acetone 〉 methanol.The highest content of total phenolics was found in water extract,but the highest content of total flavonoids was in methanol extract.In conclusion ,the antioxidant activity of the aqueous extracts from the mycelium of Xylaria schweinitzii was significant, which probably has the potential to develop as a food antioxidant.

关 键 词：薛凡尼氏炭角菌 菌丝体 抗氧化 自由基清除 总还原力 

分 类 号：TS201.2[轻工技术与工程—食品科学]