日常用胶带和标签纸上溶血细菌的分离鉴定  

Isolation and identification of hemolytic bacteria on common adhesive tape and label paper

作　　者：严美婷 董开发[1] 曲旭龙 陈卫平[1] 张超凤[1] 张凤英[1] 

机构地区：[1]江西农业大学食品科学与工程学院,江西南昌330045

出　　处：《食品工业科技》2017年第7期281-284,共4页Science and Technology of Food Industry

摘　　要：评估生活中日常用胶带和标签纸的安全风险性,并对各样本上的溶血细菌进行分离及鉴定。采集日常生活中经常用到的胶带、标签纸,无菌操作剪取一小块贴于血平板上置于35~37℃培养,用作业本、打印纸、新闻纸等常用纸张做对照,24~48 h后通过观察溶血圈的大小来判断各种样本上微生物的溶血性的强弱,据此评估该样本的安全风险性;对各种样本上微生物菌落进行分离纯化,再通过革兰氏染色、观察细胞形态、生理生化实验和16S r DNA测序对其进行菌属鉴定。结果表明,所测定的样本上的溶血菌均为芽孢杆菌属中的解淀粉芽孢杆菌(Bacillus amyloliquefaciens),且菌来源于样本的胶质层。日常用的各种胶带、标签纸都存在一定的潜在的微生物安全风险。To assess the adhesive tape and label paper security risks in daily life, and to separate and identify the hemolytic bacteria of the samples. The adhesive tape and paper label were collected in commonly daily life, and a small piece of the samples were sheared on the blood plate with aseptic technique, cultivated at 35-37 %, and the comparisons were workbook, printing paper, newsprint and other commonly used paper.The strength of the microbial hemolytic were judged by observing the size of hemolysis circle of the samples after 24-48 hours cultivating, and the safety risks of the samples were also assessd.The samples were separated and purified on microbial colony, and through the gram stain, ohserviation of cell morphology, physiological, biochemical test and 16S rDNA sequencing, the species were identificated. Results showed that the hemolytic bacterium of samples for determination is Bacillus amyloliquefaciens, and it comes from glial layer of the samples. To some degree,daily adhesive tapes, label papers have potential microbial security risks.

关 键 词：溶血性 胶带 标签纸 鉴定 解淀粉芽孢杆菌 

分 类 号：TS201.6[轻工技术与工程—食品科学]