不同食用油脂对大鼠胰岛素抵抗和糖异生的影响  

Effect of different oil on insulin resistance and gluconeogenesis in rats

作　　者：卜文婕[1,2] 李艳[2] 童丽[3] 杨建课[1] 孙敏[2] 

机构地区：[1]皖南医学院医学生物学教研室,安徽芜湖241002 [2]安徽大学生命科学学院,安徽合肥230601 [3]青海大学医学院中藏药研究中心,青海西宁810001

出　　处：《食品工业科技》2017年第7期361-364,共4页Science and Technology of Food Industry

基　　金：青海省重点研发与转化计划项目(2016-SF-126);国家自然基金面上项目(31670361);皖南医学院重点科研项目培育基金(WK2015Z04)

摘　　要：目的:比较不同油脂对SD大鼠胰岛素抵抗和糖异生的影响。方法:将雄性SD大鼠以7只为一组分为4组,对照组食用基础饲料,猪油、橄榄油和油茶籽油组大鼠食用含相应油脂高脂饲料,连续喂养17周,处死前进行口服葡萄糖耐量(OGTT)和胰岛素耐量实验(ITT),大鼠取血处死后,取血清检测空腹血糖(FPG)、空腹胰岛素(FINS)和脂联素的水平,计算胰岛素抵抗指数(HOMA-IR),并采用Real time PCR技术检测大鼠肝脏中糖异生相关基因磷酸烯醇丙酮酸羧激酶(PEPCK)、葡萄糖6-磷酸酶(G6Pase)的mRNA表达。结果:各油脂组大鼠表现出不同程度的胰岛素抵抗(IR),猪油组大鼠FPG、FINS和HOMA-IL均显著升高(p<0.05),OGTT和ITT异常,血清脂联素明显降低(p<0.05),而橄榄油和油茶籽油组大鼠IR程度显著低于猪油组(p<0.05),油茶籽油组脂联素水平明显高于猪油组(p<0.05)。糖异生相关基因中,各油脂组大鼠PEPCK和G6P表达量均升高(p<0.05),其中猪油组明显高于其他组(p<0.05),橄榄油组和油茶籽油组与正常组间差异较小,但存在统计学意义(p<0.05)。结论:富含饱和脂肪酸(SFA)的猪油可增加产生IR的风险,而不饱和脂肪酸(UFA)丰富的橄榄油和油茶籽油诱导大鼠IR程度明显低于猪油组,有统计学意义(p<0.05),其中油茶籽油作为我国特产油脂,可作为优质油脂进行食用。Objective ：To compare the effects of different oil on insulin resistance and glueoneogenesis in SD rats.Methods：Male SD rats were divided into four groups（ n = 7 ）.Rats in the control group were fed normal diet and the high fat diet groups were fed with diet containing different oil（ lard, olive, and camellia seeds oil）.Rats were fed for 17 weeks.Oral glucose tolerance test （OGTT） and insulin tolerance test （ITF）were performed before sacrificed. The artery blood samples were collected after anesthesia,then serum were separarted to detect the levels of fasting plasma glucose （FPG） , fasting serum insulin （FINS） and adiponectin.The mRNA expression of phosphoenolpyruvate carboxykinas（PEPCK） and glueose-6-phosphatase（G6P） related to gluconeogenesis in liver,were analyzed by real time PCR.Results：Rats in each oil-fed group showed different levels of insulin resistance.Lard-fed rats led to the significantly increased in levels of FPG, FINS and HOMA-IR （p 〈 0.05 ） , impaired OGTT and ITT performance. The level of serum adiponeetin was decreased in lard-fed rats compared with others （p 〈 0.05 ）.The expression of gluconeogenesis-related genes（PEPCK and G6Pase）in all oil-fed rats were higher than those in control group （p 〈0.05）. The mRNA expression in lard-fed rats were significantly higher than other groups （p 〈 0.05 ）. Olive oil and camellia seeds oil-fed rats showed little difference compared with the normal group, but there was statistical significanee（p 〈 0.05 ）.Conclusion ： Lard riched in saturated fatty acids （SFA）may increase the risk of IR.The olive and camellia seeds oil, which have great amount of unsaturated fatty acids （ UFA ）, induced significantly lower degree of IR than lard （p 〈 0.05 ）. Camellia seed oil as a characteristic oil in China, can be used as a high quality oil.

关 键 词：食用油 胰岛素抵抗 脂联素 糖异生 

分 类 号：TS201.4[轻工技术与工程—食品科学]