Neural stem cells over-expressing brain-derived neurotrophic factor promote neuronal survival and cytoskeletal protein expression in traumatic brain injury sites  

作　　者：Tao Chen Yan Yu Liu-jiu Tang Li Kong Cheng-hong Zhang Hai-ying Chu Liang-wei Yin Hai-ying Ma 

机构地区：[1]Department of Neurosurgery,the First Affiliated Hospital of Dalian Medical University [2]Department of Histology and Embryology,Dalian Medical University [3]Department of Oncology,Dalian Central Hospital

出　　处：《Neural Regeneration Research》2017年第3期433-439,共7页中国神经再生研究（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China,No.31300812 and No.31371218

摘　　要：Cytoskeletal proteins are involved in neuronal survival.Brain-derived neurotrophic factor can increase expression of cytoskeletal proteins during regeneration after axonal injury.However,the effect of neural stem cells genetically modified by brain-derived neurotrophic factor transplantation on neuronal survival in the injury site still remains unclear.To examine this,we established a rat model of traumatic brain injury by controlled cortical impact.At 72 hours after injury,2 × 10~7 cells/m L neural stem cells overexpressing brain-derived neurotrophic factor or naive neural stem cells（3 m L） were injected into the injured cortex.At 1–3 weeks after transplantation,expression of neurofilament 200,microtubule-associated protein 2,actin,calmodulin,and beta-catenin were remarkably increased in the injury sites.These findings confirm that brain-derived neurotrophic factor-transfected neural stem cells contribute to neuronal survival,growth,and differentiation in the injury sites.The underlying mechanisms may be associated with increased expression of cytoskeletal proteins and the Wnt/β-catenin signaling pathway.Cytoskeletal proteins are involved in neuronal survival.Brain-derived neurotrophic factor can increase expression of cytoskeletal proteins during regeneration after axonal injury.However,the effect of neural stem cells genetically modified by brain-derived neurotrophic factor transplantation on neuronal survival in the injury site still remains unclear.To examine this,we established a rat model of traumatic brain injury by controlled cortical impact.At 72 hours after injury,2 × 10~7 cells/m L neural stem cells overexpressing brain-derived neurotrophic factor or naive neural stem cells（3 m L） were injected into the injured cortex.At 1–3 weeks after transplantation,expression of neurofilament 200,microtubule-associated protein 2,actin,calmodulin,and beta-catenin were remarkably increased in the injury sites.These findings confirm that brain-derived neurotrophic factor-transfected neural stem cells contribute to neuronal survival,growth,and differentiation in the injury sites.The underlying mechanisms may be associated with increased expression of cytoskeletal proteins and the Wnt/β-catenin signaling pathway.

关 键 词：nerve regeneration brain-derived neurotrophic factor neural stem cells transfect differentiation traumatic brain injury CYTOSKELETON NEUROFILAMENT microtubule-associated proteins CALMODULIN Wnt/β-catenin neural regeneration 

分 类 号：R651.15[医药卫生—外科学]