紫色黄秋葵转录组功能基因测序及分析  

The Purple Abelmoschus esculentus Transcriptome as a Source for Gene Sequence Information

作　　者：张少平[1,2] 邱珊莲[1] 郑云云[1,2] 张帅[1] 吴松海[1] 何炎森[1] 郑开斌[1,2] 

机构地区：[1]福建省农业科学院亚热带农业研究所,福建漳州363005 [2]福建省农业科学院作物研究所,福建福州350013

出　　处：《核农学报》2017年第4期643-653,共11页Journal of Nuclear Agricultural Sciences

基　　金：福建省公益类科研院所专项(2016R1012-4);福建省农业科学院青年英才计划项目(YC2015-19);福建省农业科学院科技创新团队PI项目(2016PI-2)

摘　　要：黄秋葵为药食兼用型植物,富含花青素、多糖、黄酮及萜类等活性成分。为探讨黄秋葵次级代谢过程中这些活性物质合成的遗传基础,以紫色黄秋葵嫩茎叶为材料,采用Illumina Hi Seq 2500高通量转录组测序技术获得23 026 500个短读序,经组装获得42 484个Unigene,平均长度877 bp。31 931个和21 926个Unigene分别在Nr和Swiss Prot数据库有同源比对信息;Unigene与COG、KOG数据库进行比对,根据其功能各分为24类和25类;通过Pfam数据库,所注释到的20 031个Unigene分属于7 277类蛋白结构域;GO注释到18 602个Unigene分为细胞组分、分子功能及生物学过程等三大类的51个功能组,其中大量Unigene与细胞部分、细胞、催化活性、结合活性、代谢进程及细胞进程相关;根据KEGG数据库,可将7 619个Unigene定位到119个代谢途径分支,其中有1、3、35、61、13和70个Unigene分属于花色素苷、黄酮、类黄酮、N-多糖、二萜类和萜类骨架生物合成路径,它们可能参与这些活性物质的生物合成。本研究结果为后续深入开展黄秋葵花青素、黄酮类等物质代谢合成途径及相关功能基因研究奠定了基础。Okra, belonging to a group of edible and medical plants, is rich in various bioactive substances such as anthocyanin, polysaccharide, flavoniod, diterpenoid, etc. In order to explore genetic basis for the synthesis of these active substances, the transcriptome of purple Abelmoschus esculentus was sequenced by Illumina HiSeq 2500 platform which is a new generation of high-throughput sequencing technology. A total of 23 026 500 reads fragment were generated by sequencing. The reads gave 42 484 Unigenes with an average length of 877 bp. 31 931 and 21 926 Unigenes were annotated against the Nr and SwissProt databases respectively. According to COG and KOG databases, the Unigenes were further annotated and grouped into 24 and 25 functional categories, respectively. 20 031 Unigenes were annotated against Pfam database and formed 7 277 protein structural domains. 18 602 Unigenes annotated against GO database were divided into cellular component, molecular function and biological process categories with a total of 51 functional groups. Many of 18 602 Unigenes were closely related to cell part, cell, catalytic activity, binding metabolic process and cellular process. KEGG pathway analysis showed that 7 619 Unigenes could be broadly classfied into 119 classes according to their function, and some of them were involved in anthocyanin biosynthesis （1 Unigene）, flavone and flavonol biosynthesis （3）, flavonoid biosynthesis （35）, N-glycan biosynthesis （61）, diterpenoid biosynthesis （13） and terpenoid backbone biosynthesis （70）. The Unigenes sequenced and functional annotations would lay a provide the foundation for follow-up study of synthetic ways and related genes of the bioactive substances.

关 键 词：紫色黄秋葵 转录组基因 测序分析 功能注释 

分 类 号：S649[农业科学—蔬菜学]