发状念珠藻、集胞藻及小球藻胞外多糖的理化性质及氧自由基清除能力的比较  

Comparison of physicochemical property and oxygen radical absorbance capacity between Nostoc flagelliforme,Synechocystiss sp. PCC6803 and Chlorella pyrenoidosa exopolysaccharides

作　　者：蔡露阳[1] 范华[1] 焦文冬 刘宁[1] 王宁[1] 

机构地区：[1]陕西科技大学食品与生物工程学院,陕西西安710021

出　　处：《食品与发酵工业》2017年第3期73-77,共5页Food and Fermentation Industries

基　　金：国家自然科学基金青年基金(31401633)

摘　　要：提取纯化发状念珠藻、集胞藻及小球藻胞外多糖,研究其理化性质及氧自由基清除能力。采用醇沉法提取发状念珠藻、集胞藻及小球藻的胞外多糖并经透析得到纯化的发状念珠藻、集胞藻及小球藻胞外多糖EPSF、EPSJ和EPSX。对3种胞外多糖进行光谱测定、分子量测定以及氧自由基清除能力的测定,比较分析其理化性质和氧自由基清除能力。研究表明,EPSJ的得率最高,是EPSF的4.10倍、EPSX的1.84倍。3种多糖都不含有淀粉、酚羟基和硫酸基,但含有一定量的蛋白质和糖醛酸。EPSJ、EPSF、EPSX中均存在β-糖苷键,并且EPSJ和EPSX中还存在α-糖苷键。EPSF为低分子质量多聚糖,而EPSJ和EPSX为高分子质量多聚糖且组分相对均一。EPSF、EPSJ及EPSX都具有一定清除氧自由基的能力,其中EPSF最强,EPSJ次之,EPSX最小。与EPSJ和EPSX相比,发状念珠藻胞外多糖EPSF提取物产量较低,但具有更高的氧自由基清除能力。!The physicochemical property and oxygen radical absorbance capacity of Nostocflagelliforme, Synechocystiss sp. PCC6803 and Chlorella pyrenoidosa exopolysaccharides were studied in this paper. The extracellular polysaccharides of Nostoc flagelliforme, Synechocystiss sp. PCC6803 and Chlorella pyrenoidosa （abbreviated as EPSF, EPSJ and EPSX） were precipitated by ethanol and then dialyzed. The physicochemical properties and oxygen radical absorbance capacity of EPSF, EPSJ and EPSX were studied through spectrum analysis and determination of molecular weight and oxygen radical absorbance capacity. The results showed that the yield of EPSJ was highest, which was 4. 10 times higher than that of EPSF and 1.84 times higher than that of EPSX. There were not starch, phenolic hydroxyl groups or sulfate groups in three exopolusaccharides but there were protein and uronic acid in them. EPSF, EPSJ and EPSX had β-glucosidic bonds and EPSJ and EPSX also had α-glucosidic bonds. EPSF was low molecular weight poly- saccharide. EPSJ and EPSX were high molecular weight polysaccharide and the components were homogeneous. EPSF, EPSJ and EPSX could absorb oxygen radical. EPSF was the best, and EPSJ was better than EPSX. The yield of EPSF was lower than that of EPSJ and EPSX, but EPSF possessed the best oxygen radical absorbance capacity among them.

关 键 词：发状念珠藻 集胞藻 小球藻 胞外多糖 

分 类 号：TS201.2[轻工技术与工程—食品科学]