双酶法催化玉米淀粉制备γ-CD  

γ-CD Catalyzed from Maize Starch by Dual Enzymatic Reaction

作　　者：李林林[1,2] 张梦柯[1,2] 金征宇[1,2] 王丽华[1,2] 王金鹏[1,2] LI Linlin ZHANG Mengke JIN Zhengyu WANG Lihua WANG Jinpeng(State Key Laboratory of Food Science and Technology, Wuxi 214122,China School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [2]江南大学食品学院,江苏无锡214122

出　　处：《食品与生物技术学报》2017年第4期357-363,共7页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31230057;31401524);江苏省自然科学基金项目(BK20140143);江苏省科技支撑计划项目(BE2013311)

摘　　要：以玉米淀粉为底物,研究了来自于栖热水生菌的4α-糖基转移酶(4αGTase)和来自于嗜碱芽孢杆菌的环糊精葡萄糖基转移酶(CGTase)作用于淀粉制备γ-CD的影响因素。结果表明:两种酶的添加方式为先加入4αGTase、再加入CGTase;γ-CD的最佳制备条件为底物质量分数5%,4αGTase加酶量4 U/g淀粉,CGTase加酶量8U/g淀粉,反应时间30 h。在此条件下γ-CD的得率最高为12.83%,比对照组提高了76.7%。Theconjunction of 4α-glycosyltransferase （4aGTase） with cyclodextrin glycosyltransferase （CGTase） were screened for their ability o^producing γ-cyclodextrin （CD） usingmaize starch as substrate. The studies on the influence factors showed that 4aGTase should be added first,followed byCGTase. The optimal conditions for γ-CD production was as following ：4U of 4αGTase and 8U ofCGTase per gram of substratewere used to convert maize starch（5%, w/ y） into 7-CD with the reaction time of 30 h. the subsequent yield of γ-CD was 12.83%, which was 76.7% higher than the control. This study has potential value for the industrial production of γ -CD.

关 键 词：4α-糖基转移酶 环糊精葡萄糖基转移酶 Γ-环糊精 玉米淀粉 

分 类 号：TS236.9[轻工技术与工程—粮食、油脂及植物蛋白工程]