翡翠贻贝糖蛋白提取工艺优化及体外抗氧化活性  

On Extraction Optimization and Antioxidant Activity of Glycoprotein from Pernaviridis

作　　者：刘淑集[1,2,3] 潘裕添[4] 刘智禹[2,3] 王茵 苏永昌[2,3] 刘秋凤[2,3] 陈锦权[1] LIU Shu-ji PAN Yu-tian LIU Zhi-yu WANG Yin SU Yong-chang LIU Qiu feng CHEN Jin-quan(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China Fisheries Research Institute of Fujian , National Research and Development Center for Marine Fish Processing （Xiamen） ,Key Laboratory of Cultivation and High - value Utilization of Marine Organisms in Fujian Province, Xiamen Fujian 361013 . China Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen Fujian 361013 , China Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou Fujian 363000 , China)

机构地区：[1]福建农林大学食品科学学院,福州350002 [2]福建省水产研究所,国家海水鱼类加工技术研发分中心(厦门),福建省海洋生物增养殖与高值化利用重点实验室,福建厦门361013 [3]福建省海洋生物资源开发利用协同创新中心,福建厦门361013 [4]闽南师范大学菌物工程技术研究中心,福建漳州363000

出　　处：《西南师范大学学报（自然科学版）》2017年第4期45-52,共8页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：福建省省属公益类科研院所基本科研专项(2014R1003-9);福建省科技重大专项(2014NZ0001-1;14PZY017NF17);厦门市科技专项(3502Z20132002);国家海洋公益性行业科研专项(201405016-1;2014FJPT01)

摘　　要：以翡翠贻贝肉为原料,在单因素试验的基础上,通过响应面试验设计优化了翡翠贻贝糖蛋白提取的工艺条件.采用超滤方法获得翡翠贻贝超滤截留液糖蛋白PGFR(Pernaviridis glycoprotein of the filter remains of MW cut off 5KDa)和翡翠贻贝超滤透过液糖蛋白PGFL(Pernaviridis glycoprotein of the filter leaching of MW cut off 5KDa)2个组分,并分别评价其体外抗氧化活性.结果表明,从翡翠贻贝肉中提取糖蛋白粗提物的最佳工艺条件为提取时间61min,提取温度62℃,NaCl浓度0.2mol/L,料液比1∶1,在该条件下提取率为51.55%.体外抗氧化试验结果表明,翡翠贻贝PGFR对·OH,·O_2^-和DPPH自由基的清除率及总抗氧化能力均优于PGFL,可作为后续研究的主要对象,具有开发新型天然抗氧化剂的潜力.The optimum extraction technology and antioxidant activity of glycoprotein from Pernaviridis have been performed for future development and utilization of the Pernaviridis.On the basis of single-factor experiment,the optimum parameters of extraction for glycoprotein fromPernaviridis have been determined by response surface methodology(RSM).2fractions PGFR(Pernaviridis glycoprotein of the filter remains of MW cut off 5KDa)and PGFL(Pernaviridis glycoprotein of the filter leaching of MW cut off5KDa)have been obtained by ultra-filtration.Then,antioxidant activities of 2fractions have been examined by using various antioxidant assays with vitamin C co-assayed as positive control in vitro.Results show that the optimized condition of glycoprotein extraction was as follows:extraction temperature of62℃,extraction time of 61 min,NaCl concentration of 0.2mol/L,ratio of solid to liquid of 1∶1.At the optimum conditions,the yield of saccharide and protein reached to 51.55%.The results of above antioxidant tests demonstrate that PGFR had higher scavenging effect of·OH,·O_2^-and DPPH free radicals and total antioxidant activities than PGFL,which indicating that PGFR would be a good potential source for natural antioxidant and worth a subsequent research.

关 键 词：翡翠贻贝 糖蛋白 提取 超滤 抗氧化 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]