芍药花多酚的纯化及其抗氧化活性研究  

Study on Purification and Antioxidant Activity of Polyphenols from the Flower of Paeonia lactiflora Palla

作　　者：俞憬 陈冠林 杨璐齐 王煜坤[1] 高永清[1] 傅南琳[3] YU Jing CHEN Guan-lin YANG Lu-qi WANG Yu-kun GAO Yong-qing FU Nan-lin(School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528458, Guangdong, China Foshan Institute of Occupational Disease Prevention and Control, Foshan 528000, Guangdong, China The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510008, Guangdong, China)

机构地区：[1]广东药科大学食品科学学院,广东中山528458 [2]佛山市职业病防治所,广东佛山528000 [3]广东药科大学附属第一医院,广东广州510008

出　　处：《食品研究与开发》2017年第7期38-44,共7页Food Research and Development

基　　金：广东省医学科学技术研究基金项目(A2015617);中医药强省建设专项资金第二批名中医师承项目(粤中医办函[2015]93号)

摘　　要：研究大孔吸附树脂纯化芍药花多酚的条件和纯化后多酚的抗氧化能力。采用静态试验和动态试验确定纯化条件,计算纯化后多酚的含量。在供试的9种大孔吸附树脂中,HPD100树脂的吸附和洗脱效果最好。在室温下,以50%乙醇为洗脱剂,吸附流速和洗脱流速均为10 m L/min时,纯化的芍药花多酚含量为721.72 mg GAE/g DW,是未纯化前的1.57倍。通过DPPH、FRAP和ABTS三种方法测得纯化后芍药花多酚的抗氧化能力是未纯化前粗提物的1.35、1.44、1.62倍。Using the macroporous adsorptive resins, the purification conditions for total phenolic content（TPC） from the flower of Paeonia lactiflora Palla and the antioxidant capacity of the TPC were studied after purification. Static and dynamic experimental methods were performed to determine the purification conditions, and the con-tents of the purified TPC were calculated. Among the 9 macroporous resins tested, HPD100 resin was the best in adsorption effect and desorption effect. At room temperature, when 50%of ethanol was used as desorption solu-tion, adsorption and desorption flow velocities were both 10 mL/min, the content of the TPC purified from the flower of Paeonia lactiflora Palla was 721.72 mg GAE/g dry weight, and it was 1.57 times higher compared with that before purification. The antioxidant activities of purified extracts measured by 2,2-diphenyl-1-picrylhy-drazyl radical scavenging activity assay（DPPH）, ferric reducing antioxidant power assay（FRAP）, and ABTS＋radical scavenging activity （ABTS） assay were 1.35, 1.44 ,1.62 times higher than those of crude extracts before purification, respectively.

关 键 词：纯化 抗氧化 多酚 芍药花 大孔树脂 

分 类 号：TQ464[化学工程—制药化工]