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作 者:江涛[1] 韩春卉[1] 张宏元[1] 张靖[1] 王佳慧[1] 李楠[1] 吕涵阳 李凤琴[1] JIANG Tao HAN Chun-hui ZHANG Hong-yuan ZHANG Jing WANG Jia-hui LI Nan LYU Han-yang LI Feng-qin(Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100021, China)
机构地区:[1]国家食品安全风险评估中心卫生部食品安全风险评估重点实验室,北京100021
出 处:《中国食品卫生杂志》2017年第2期126-130,共5页Chinese Journal of Food Hygiene
基 金:北京市自然科学基金(5141002)
摘 要:目的针对北京市市售牡蛎样品中诺如病毒污染水平及污染浓度进行定量分析研究。方法分离牡蛎消化腺,将消化腺匀浆处理,加入含有蛋白酶K的磷酸盐缓冲液,进行样品前处理,用试剂盒提取病毒RNA,用一步法实时荧光逆转录聚合酶链式反应(real time RT-PCR)检测诺如病毒RNA,并对阳性样品进行定量分析。结果共检测牡蛎样品356份,其中GGI阳性样品12份,GGII阳性样品39份,GGI和GGII同时为阳性的样品6份。对阳性样品中的诺如病毒核酸定量分析,核酸浓度在3.7×10~3~2.8×10~5基因拷贝/g(消化腺)之间。结论北京市市售牡蛎中存在诺如病毒污染的情况,需要加强对牡蛎中诺如病毒的污染监测,并开展污染水平风险评估,保障消费者食用安全,降低由诺如病毒引起的腹泻病的疾病负担。Objective The project was carried out to study the contamination levels of Norovirus in oysters in Beijing city.Methods The Norovirus was extracted from digestive glands by phosphate buffer containing proteinase K.The RNA was extracted and purified with virus RNA extraction kit.Norovirus RNA was detected using Taqman-based one-step real time reverse transcription-polymerase chain reaction(real time RT-PCR) and quantitative analysis was performed.Results Three hundred and fifty-six oyster samples were detected.Fifty-seven samples were positive,including twelve GGI positive samples alone,thirty-nine GGII positive samples alone and six samples of GGI and GGII positive both.The range of the quantity of Norovirus in positive samples was 3.7 × 10~3-2.8 × 10~5 gene copies/g(digestive gland).Conclusion The oysters sold in Beijing were contaminated by Norovirus.The monitoring of Norovirus in oysters should be strengthened and risk assessment of Norovirus should be carried out to ensure safety and health of consumers and reduce the burden of disease caused by norovirus that causes diarrhea.
关 键 词:诺如病毒 牡蛎 实时荧光逆转录聚合酶链式反应 核酸 检测 食品安全 食品污染物
分 类 号:R155.5[医药卫生—营养与食品卫生学]
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