高效液相色谱-电感耦合等离子体质谱联用技术测定富硒食品中无机硒和有机硒的含量  

Determination of inorganic and organic selenium in food by high performance liquid chromatography-inductively coupled plasma mass spectrometry

作　　者：张颖[1] 杨清清[1] 宋毅[1] 闻胜[1] 黄文耀[1] ZHANG Ying YANG Qing-qing SONG Yi WEN Sheng HUANG Wen-yao(Hubei Provincial Key Laboratory for Applied Toxicology, Institute of Health Inspection and Testing, Hubei Provincial Center for Disease Control and Prevention, Hubei Wuhan 430079, Chin)

机构地区：[1]湖北省疾病预防控制中心卫生检验检测研究所应用毒理湖北省重点实验室,湖北武汉430079

出　　处：《中国食品卫生杂志》2017年第2期181-185,共5页Chinese Journal of Food Hygiene

摘　　要：目的建立高效液相色谱-电感耦合等离子体质谱联用技术(HPLC-ICP/MS)测定富硒食品中无机硒和有机硒含量的方法。方法采用纯水提取富硒食品中的无机硒,通过透析作用分离小分子量的硒化合物与样品基体,利用阴离子交换色谱分离,采用Hamilton PRP-X100分析柱和预柱(250 mm×4 mm,10μm),流动相为5 mmol/L柠檬酸铵中加2%甲醇,电感耦合等离子体质谱仪测定样品中无机硒的含量。根据总硒与无机硒的差值计算有机硒含量。结果硒(IV)和硒(VI)检出限分别为6.67和9.95μg/kg,二者回收率分别为70.2%~108.1%和73.2%~116.9%。结论该方法可满足富硒食品中无机硒和有机硒含量的测定。Objective To establish a method for the determination of inorganic and organic selenium in food by high performance liquid chromatography-inductively coupled plasma mass spectrometry（HPLC/ICP-MS）.Methods Inorganic selenium was extracted by ultrapure water.Dialysis effect was used to remove sample matrix,inorganic selenium was separated and detected by anion exchange chromatography-inductively coupled plasma mass spectrometry.Results The detection limit of Se（IV） and Se（VI） were 6.67 and 9.95 μg/kg,the recoveries were 70.2%-108.1% and73.2%-116.9%.Conclusion This method could be used for the determination of inorganic selenium and organic selenium in selenium-enriched food.

关 键 词：高效液相色谱法 电感耦合等离子体质谱法 硒 微量元素 形态 测定 食品 富硒食品 

分 类 号：R155.5[医药卫生—营养与食品卫生学]