HS-SPME/GC-MS法分析榅桲果实中的挥发性成分  

Analysis of volatile components in quince (Cydonia oblonga Mill.var.maliformis) fruit by head-space solid-phase micro-extraction/gas chromatography-mass spectrometry

作　　者：哈及尼沙[1] 古丽巴哈尔.卡吾力 阿米乃木.买买提 王强[2] 

机构地区：[1]新疆医科大学药学院,乌鲁木齐830011 [2]新疆大学理化测试中心,乌鲁木齐830046

出　　处：《西北药学杂志》2017年第3期263-266,共4页Northwest Pharmaceutical Journal

基　　金：国家自然科学基金项目(编号:31360078)

摘　　要：目的建立分析榅桲新鲜果实中挥发性成分的方法。方法利用顶空-固相微萃取技术(HS-SPME)提取榅桲果实中的挥发性成分,采用气相色谱-质谱联用技术(GC-MS)结合保留指数法分离分析榅桲果实中的挥发性成分,并用峰面积归一化法测定相对百分含量。结果从榅桲果实的挥发油中分离并鉴定出27个化合物,占相对总含量的99.85%,其主要成分有金合欢烯(38.92%)、辛酸乙酯(19.44%)、己酸乙酯(6.52%)、顺3-乙酸叶醇酯(6.45%)和茶螺烷(6.02%)等。结论 HS-SPME技术提取挥发油是无需溶剂的快速、简单、有效的方法,用GC-MS结合保留指数获得的结果更准确。这种方法适用于榅桲新鲜果实中挥发性成分的快速分析和质量控制。Objective To analyze the aroma constituents of the volatile oil from the quince（Cydonia oblonga Mill.var.maliformis）fresh fruit.Methods The volatile components were extracted by head-space solid-phase micro-extraction（HS-SPME）,and analyzed by GC-MS combined with retention index.The components were quantitatively determined with normalization method.Results Totally twenty-seven constituents were accurately identified,constituting for 99.85% of the total volatile oil.The main components were farnesene（38.92%）,ethyl caprylate（19.44%）,ethyl hexanoate（6.52%）,cis-3-hexenyl-1-acetate（6.45%）,theaspirane（6.02%）etc.Conclusion HS-SPME is a rapid,simple,and efficient method to collect volatile compounds in quince fruits.The results obtained by GC-MS combined with retention index were accurate.This method is applicable for rapid analysis and quality control of the volatile components of quince fruits.

关 键 词：榅桲果实 挥发性成分 顶空-固相微萃取 气相色谱-质谱联用 保留指数 

分 类 号：R284[医药卫生—中药学]