响应面法优化嗜酸乳杆菌发酵棉粕工艺条件  

Optimization for cottonseed meal fermentation by Lactobacillus acidophilus by using response surface method

作　　者：张晓羊[1] 张文举[1] 王永强[1] 杨亮[1] 王展鹏[1] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832003

出　　处：《黑龙江畜牧兽医》2017年第5期139-141,146,294,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31360564);新疆研究生科研创新项目(XJGRI2014058)

摘　　要：为了筛选嗜酸乳杆菌固体发酵棉粕的最佳产酸发酵条件,试验采用单因素试验,根据响应面的基本原理,以发酵饲料中乳酸含量为优化指标,利用Design Expert软件对发酵条件(接种量、发酵、温度和时间)进行优化。结果表明:理论最佳发酵条件是接种量为5.96 m L,发酵温度为36.99℃,发酵时间为48.26 h;此条件下发酵产物中乳酸含量预测可达7.57%,实际含量为7.50%。To optimize the fermentation conditions for lactic acid production of Lactobacillus acidophilus, one - factor - at - a - time designs and response surface method were introduced to the current study. The one - factor - at - a - time designs indicated that incoculum amount, temper- ature and fermented time exhibited a significant influence on cottonseed meal fermentation, respectively. Furthermore, the fermentation conditions for lactic acid production in the fermented feed was optimized by the expert design software according to the basic principle of the response surface method. Results revealed that theoreticaUy, the optimal fermentation conditions were as follows： incoculum amount 5.96 mL, fermentation temperature 36.99℃ and fermented time 48.26 h ; the maximum lactic acid production under the optimized conditions was 7.50% instead of 7.57% theoretically.

关 键 词：棉粕 发酵 嗜酸乳杆菌 单因素试验 响应面法 最佳发酵条件 

分 类 号：S816.43[农业科学—饲料科学] TS202.3[农业科学—畜牧学]