窖泥中产丁酸菌的筛选、鉴定及生长性能研究  

Isolation and Identification of a Butyric Acid-Producing Strain from Pit Mud and Study on Its Growth Performance

作　　者：袁华伟[1] 赵猛[1] 蒋君[1] 刘光钱 胡永奇[2,3] 沈才洪[2,3] 吴重德[4] 周荣清[3,4] 宋川[2,3] 

机构地区：[1]宜宾学院生命科学与食品工程学院,四川宜宾644000 [2]泸州老窖股份有限公司,四川泸州646000 [3]国家固态酿造工程技术研究中心,四川泸州646000 [4]四川大学轻纺与食品学院,四川成都610065

出　　处：《酿酒科技》2017年第5期35-40,共6页Liquor-Making Science & Technology

基　　金：四川省科技厅科技计划重点项目<基于风味指纹图谱的微生物强化共培技术研究及应用>(2014SZ0129);四川省科技成果转化项目<浓香型白酒智能化与自动化生产关键技术集成及产业化示范>(2016CC0032)

摘　　要：通过对川南某酒厂的老窖泥富集培养、分离纯化,经丁酸发酵实验,筛选出1株高产丁酸的梭菌。经16Sr DNA鉴定,该菌为丁酸梭状芽孢杆菌(Clostridium butyricum),命名为丁酸菌YD-4。经对该菌株生长性能条件研究,其最适生长温度为37℃,最适p H值为7.0,最适接种量为2%,菌体浓度达1.64×108个/m L,产丁酸量可达到3.44 g/L。A Clostridium strain with high yield of butyric acid was isolated through cumulation cultivation, isolation, purification, and butyric acid fermentation from aged pit mud in a distillery in south Sichuan. 16Sr DNA identification suggested that such strain is Clostridium butyricum and it was named YD-4. The research on its growth performance indicated that its optimum growth tempera- ture was at 37℃, its optimum pH value was about 7.0, its best inoculating quantity was 2 %. As the total number of bacteria reached up to 1.64× 10^8个/mL, the yield of butyric acid was 3.44 g/L.

关 键 词：窖泥 丁酸菌 筛选 鉴定 生长性能 

分 类 号：TS261.1[轻工技术与工程—发酵工程]